Evaluation of the effects of gonadotropin-relasing hormone antagonist (GnRH-ant) and agonist (GnRH-a) in the prevention of postoperative adhesion formation in a rat model with immunohistochemical analysis

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dc.contributor.authorTamay A.G.
dc.contributor.authorGuvenal T.
dc.contributor.authorMicili S.C.
dc.contributor.authorYildirim Y.
dc.contributor.authorOzogul C.
dc.contributor.authorKoyuncu F.M.
dc.contributor.authorKoltan S.O.
dc.date.accessioned2024-07-22T08:19:56Z
dc.date.available2024-07-22T08:19:56Z
dc.date.issued2011
dc.description.abstractObjective: To investigate the effects of GnRH antagonist (GnRH-ant) and agonist (GnRH-a) in the prevention of postoperative pelvic adhesions by a visual scoring system and immunohistochemical methods in a rat uterine horn model. Design: Controlled experimental animal study. Setting: Animal laboratory at an academic research environment. Animal(s): Twenty-one Wistar albino rats. Intervention(s): Rats were randomized into three groups. One week before the operation the rats received either GnRH-ant or GnRH-a or saline solution; they then underwent surgical laparotomy, and both uterine horns were traumatized by a scalpel. Three weeks later, all rats were sacrificed and extension and severity of the adhesions in each group were scored by a visual scoring system. Adhesion tissues were evaluated immunohistochemically for vitronectin and u-PAR. Main Outcome Measure(s): Scores of extend and severity of adhesions and staining of vitronectin and u-PAR. Result(s): The extent of adhesion scores were 1.85 ± 0.86, 0.78 ± 1.05, and 0.42 ± 0.64, and the severity of adhesion scores were 1.71 ± 0.91, 0.57 ± 0.85, 0.50 ± 0.75 for control, GnRH-ant, and GnRH-a groups, respectively. The extent and severity of adhesions were significantly lower in both GnRH-ant and GnRH-a groups when compared with the control group. Adhesion extent scores in the GnRH-a group were lower than in the GnRH-ant group, but this difference was not significant. vitronectin and u-PAR staining were significantly greater in both the GnRH-ant and GnRH-a groups than in the control group. Conclusion(s): GnRH-ant as well as GnRH-a reduced postoperative adhesion formation in a rat model. This finding was supported immunohistochemically by vitronectin and u-PAR staining. © 2011 American Society for Reproductive Medicine.
dc.identifier.DOI-ID10.1016/j.fertnstert.2011.09.001
dc.identifier.issn15565653
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/17915
dc.language.isoEnglish
dc.rightsAll Open Access; Bronze Open Access
dc.subjectAnimals
dc.subjectFemale
dc.subjectFertility Agents, Female
dc.subjectGonadotropin-Releasing Hormone
dc.subjectHormone Antagonists
dc.subjectImmunohistochemistry
dc.subjectLeuprolide
dc.subjectPostoperative Complications
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectReceptors, Urokinase Plasminogen Activator
dc.subjectSeverity of Illness Index
dc.subjectTime Factors
dc.subjectTissue Adhesions
dc.subjectUterine Diseases
dc.subjectUterus
dc.subjectVitronectin
dc.subjectcetrorelix
dc.subjectleuprorelin
dc.subjecturokinase
dc.subjectvitronectin
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectarticle
dc.subjectcontrolled study
dc.subjectdisease severity
dc.subjectdrug effect
dc.subjectfemale
dc.subjectimmunohistochemistry
dc.subjectlaparotomy
dc.subjectnonhuman
dc.subjectpelvic disease
dc.subjectpostoperative complication
dc.subjectpostoperative pelvic adhesion
dc.subjectpriority journal
dc.subjectrandomized controlled trial
dc.subjectrat
dc.subjectscoring system
dc.subjectvisual scoring system
dc.titleEvaluation of the effects of gonadotropin-relasing hormone antagonist (GnRH-ant) and agonist (GnRH-a) in the prevention of postoperative adhesion formation in a rat model with immunohistochemical analysis
dc.typeArticle

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