Investigation of Poly-β-Hydroxybutyrate (PHB) Production by Bacillus subtilis ATCC 6633 Under Different Conditions
No Thumbnail Available
Date
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The aim of our study was to assess the PHB productivity of Bacillus subtilis ATCC 6633 strain under different conditions. PHB amount was measured by UV-VIS spectrophotometer at 235 nm. The effects of the incubation time, temperature, pH, carbon sources, nitrogen sources and the rate of carbon/nitrogen ratio on PHB production rate were investigated. The results demonstrated that the highest PHB production was obtained after 24 h of incubation (10.4981 mu g/ml), at 7.0 of pH (10.4981 mu g/ml), with D-mannitole (23.6623 mu g/ml) as carbon source, L-glycine (14.6217 mu g/ml) as nitrogen source, C/N ratio of 2.5 (3.2481 mu g/ml) and with a temperature of 30 degrees C in culture media (10.4981 mu g/ml).