Effects of sevofluran on cell division and levels of sister chromatid exchange; [Die wirkung von sevofluran auf zellteilung, mitose-index (MI) und austausch der schwesterchromatide (sister chromatide exchange SCE)]

Lüleci N.; Sakarya M.; Topçu I.; Lüleci E.; Erinçler T.; Solak M.

Effects of sevofluran on cell division and levels of sister chromatid exchange; [Die wirkung von sevofluran auf zellteilung, mitose-index (MI) und austausch der schwesterchromatide (sister chromatide exchange SCE)]

Date

2005

Authors

Abstract

Objective: Purpose of the study was to investigate the mitotic index (MI) and sister chromatid exchange (SCE) levels to identify the mutagenic and carcinogenic effects of sevoflurane (sevoflurane). Methods: 42 non-smoking male and female turkish patients of ASA-risk I and II were included. The patients received an anaesthesia induction with 8% sevoflurane in 100% oxygen ("tidal volume methode") and 0,1 mg/kg BW vecuronium for neuromuscular block and endotracheal intubation. Anaesthesia was maintained with 2.0-2.5 sevoflurane in 60% N2O and 40% O2. Four 5 ml venous blood samples werde taken: before induction (control), 60 minutes, 24 hours and 5 days after sevoflurane anesthesia. Samples were prepared according to the periferic blood culture assay, modified by Morhead and co-workers, and levels of MI and SCE were examined. Results: 60 minutes after sevoflurane-anaesthesia a significant decrease of MI was found compared to controls (p < 0.01). This depression was lower after 24 hours (p < 0.05) and reversible after 5 days. SCE increased significantly during 60 minutes of anaesthesia (p < 0.001), was also lower after 24 hours (5.6 ± 2.4 vs. 4.4 ± 1.7) and returned to normal levels after 5 days (p > 0.05). Conclusion: The application of sevoflurane for anaesthesia may influence the cell division in humans and may have a mutagenic effect on DNA at the cell level, which is reversible.