Role of a combination dietary supplement containing mucopolysaccharides, vitamin C, and collagen on tendon healing in rats

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dc.contributor.authorGemalmaz, HC
dc.contributor.authorSanyilmaz, K
dc.contributor.authorOzkunt, O
dc.contributor.authorGurgen, SG
dc.contributor.authorSilay, S
dc.date.accessioned2024-07-18T11:51:18Z
dc.date.available2024-07-18T11:51:18Z
dc.description.abstractObjective: The aim of this study was to investigate the effect of mucopolysaccharide, vitamin C, and collagen supplementation on the healing of Achilles tendon in rats. Methods: Sixteen rats were separated into 2 groups. Both Achilles tendons of all rats were transected 5 mm above the insertion and repaired using a Kessler suture. After the surgical repair, the study group received the daily recommended amount of the supplement by gastric gavage, while the control group received a placebo. At the end of the third week, the animals were sacrificed. The biomechanical properties of the groups were compared with ultimate tensile strength and stiffness tests. The biological properties of the 2 groups were assessed with a histomorphometric comparison to determine the amount of collagen type I (COL1), proliferating cell nuclear antigen (PCNA), and transforming growth factor beta 1 (TGF-beta 1) expression in 3 different tissue subgroups (collagen matrix, tenocytes, and endotenon fibroblasts). Results: Analysis of histomorphometric results revealed that the rats receiving dietary supplements demonstrated a significant increase in PCNA (mean value of 86 in the control group and 168.85 in the trial group; p < 0.05) and TGF-beta 1 (mean value of 87.57 in the control group and 161.85 in the trial group; p < 0.05) in the endotenon fibroblasts of the repair site. However, there was no difference between the groups in PCNA or TGF-beta 1 when the collagen matrix and the tenocytes of the repair site were examined. Furthermore, no significant difference could be found between groups in COL1 in any of the 3 tissue subgroups (collagen matrix, tenocytes, and endotenon fibroblasts). The statistical analysis also indicated that the rats receiving supplements did not demonstrate a significant increase in the ultimate tendon tensile strength or stiffness. Conclusion: The results of this study revealed no advantage to the oral administration of the trial supplement in collagen synthesis or biomechanical properties in rats after 3 weeks using the presented study design. However, the increased expression of PCNA and TGE-beta 1 seen in the endotenon fibroblasts of the repair site might play a role in the continuum of tendon healing. (C) 2018 Turkish Association of Orthopaedics and Traumatology. Publishing services by Elsevier B.V.
dc.identifier.issn1017-995X
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/4766
dc.language.isoEnglish
dc.publisherTURKISH ASSOC ORTHOPAEDICS TRAUMATOLOGY
dc.subjectGROWTH-FACTOR-BETA
dc.subjectI COLLAGEN
dc.subjectDOUBLE-BLIND
dc.subjectTENOCYTES
dc.subjectGLUCOSAMINE
dc.subjectMETHYLSULFONYLMETHANE
dc.subjectPROLOTHERAPY
dc.subjectMECHANISMS
dc.subjectTHERAPY
dc.subjectDISEASE
dc.titleRole of a combination dietary supplement containing mucopolysaccharides, vitamin C, and collagen on tendon healing in rats
dc.typeArticle

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