Attenuation of Docetaxel-induced Oxidative Stress and Apoptosis in HEK 293 Human Embryonic Kidney Cells by Curcumin Treatment
| dc.contributor.author | Suleyman İLHAN | |
| dc.date.accessioned | 2024-07-24T09:07:54Z | |
| dc.date.available | 2024-07-24T09:07:54Z | |
| dc.date.issued | 2021 | |
| dc.description.abstract | Docetaxel (DOC) is a chemotherapeutic that induces microtubule stabilization. It is often used in breast,prostate, lung and gastric cancers but severe side effects such as cardiotoxicity, neurotoxicity,hepatotoxicity, and nephrotoxicity limit its usage. Curcumin (CUR), a natural bioactive compoundderived from turmeric. Here, the possible preventive effect of CUR against DOC-induced oxidative stressand apoptosis on HEK-293 immortalized human embryonic kidney cells. Viability was assessed via MTTassay. The generation of ROS was measured by CM-H2DCFDA dye. Phosphatidylserine externalizationand caspase 3/7 activity were used to measure apoptosis. CUR pretreatment remarkably inhibited DOCinduced cell viability reduction, ROS generation, and cell apoptosis in HEK-293 cells. Moreover, thisstudy revealed that CUR pretreatment decreased caspase-3 activity. Thus, this study highlights the novelpharmacological mechanisms of CUR and understanding the detailed mechanisms of CUR action. | |
| dc.identifier.DOI-ID | 10.18466/cbayarfbe.752495 | |
| dc.identifier.issn | 1305-130X | |
| dc.identifier.uri | http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/20913 | |
| dc.language.iso | eng | |
| dc.title | Attenuation of Docetaxel-induced Oxidative Stress and Apoptosis in HEK 293 Human Embryonic Kidney Cells by Curcumin Treatment | |
| dc.type | Araştırma Makalesi |