Preparation of a 99mTc-labeled graft polymer and its in vitro and in vivo evaluation
No Thumbnail Available
Date
2021
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The aim of this study is the synthesis of a novel 99mTc-labeld graft polymer and the biological evaluation of its in vitro and in vivo properties. To this end, a L-proline-graft-poly(HEMA) was prepared and labeled with 99mTc. The radiochemical yield of approximately the 99mTc-labeled compound amounted to 97 ± 2.3%. The cytotoxicity test revealed no cytotoxic effect after a 24- and 48-h incubation. The results of the hemolysis test showed that hemolysis was non-toxic with an effect level of less than 2%. Subsequently, the biodistribution in healthy rats was determined. High accumulation of the polymer was observed in the pancreas, thyroid and prostate. © 2021, Akadémiai Kiadó, Budapest, Hungary.
Description
Keywords
polymer , technetium 99m , animal tissue , Article , chemical structure , controlled study , cytotoxicity , embryo , HEK293 cell line , hemolysis assay , human , human cell , in vitro study , in vivo study , incubation time , lipophilicity , male , nonhuman , pancreas , prostate , radiolabeling , rat , synthesis , thyroid gland , Wistar rat