Proapoptotic and Anticancer Potentials of Thymus capitatus Essential Oil on Colon Cancer Stem Cells

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dc.contributor.authorDudu Özkum YAVUZ
dc.contributor.authorEda BECER
dc.contributor.authorRemziye KENDİRİCİ
dc.contributor.authorFeyzan Özdal KURT
dc.contributor.authorH. Seda VATANSEVER
dc.date.accessioned2024-07-24T09:10:44Z
dc.date.available2024-07-24T09:10:44Z
dc.date.issued2021
dc.description.abstractBACKGROUND/AIMS The aim of this study was to investigate the proapoptotic activity of Thymus capitatus essential oil in either colon cancer stem (CD133þ Colo-320) or nonstem (CD133– Colo-320) cells. MATERIAL and METHODS T. capitatus essential oil was obtained by water distillation and analyzed by GC–MS. Cancer stem cells (CD133þ Colo-320) were obtained from the Colo-320 cells by the MiniMACS system. Proapoptotic activity of T. capitatus essential oil was investigated by immunocytochemistry using antibodies directed against caspase-3 and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. RESULTS Caspase-3 immunoreactivity was significantly increased in 0.5% dilution T. capitatus essential oil-treated Colo-320 cells for 48 hours. Moreover, the number of TUNEL positive cells was significantly higher in Colo-320 cells when compared with CD133þ and CD133– Colo320 cells. CONCLUSION We conclude that T. capitatus essential oil increases caspase-3 molecules, which play a crucial role in apoptosis. Interestingly, T. capitatus essential oil is found to be more effective in Colo-320 cells than CD133þ Colo-320 and CD133– Colo-320 cells in terms of apoptosis.
dc.identifier.DOI-ID10.5152/cjms.2021.2293
dc.identifier.issn2149-7893
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/23138
dc.language.isoeng
dc.titleProapoptotic and Anticancer Potentials of Thymus capitatus Essential Oil on Colon Cancer Stem Cells
dc.typeAraştırma Makalesi

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