Analysis of artemisinin by a packed-column supercritical fluid chromatography-atmospheric pressure chemical ionisation mass spectrometry technique

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dc.contributor.authorDost, K
dc.contributor.authorDavidson, G
dc.date.accessioned2024-07-18T12:00:11Z
dc.date.available2024-07-18T12:00:11Z
dc.description.abstractA packed-column supercritical fluid chromatography-atmospheric pressure chemical ionisation mass spectrometry method was studied for the determination of artemisinin from Artemisia annua L. extracts. The technique does not require any kind of derivatisation prior to the analysis. All samples were simply dissolved in methanol and injected into the mobile phase. Detection was achieved by using mass spectrometry with atmospheric pressure chemical ionisation. The ionisation technique is relatively soft and provides protonated molecular ion and informative structural fragmentation for the compound. Benzophenone was used as a chromatographic standard for the determination of the analytical reproducibility. The supercritical carbon dioxide mobile phase used in the system was modified by 10% methanol. The average absolute retention time was 3.54 min with a standard deviation of 0.017 min and a relative standard deviation of 0.4% with respect to benzophenone for the procedure. The correlation coefficient was 0.998 and detection limit 370 pg on column.
dc.identifier.issn0003-2654
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/7544
dc.language.isoEnglish
dc.publisherROYAL SOC CHEMISTRY
dc.subjectPERFORMANCE LIQUID-CHROMATOGRAPHY
dc.subjectCRUDE PLANT-EXTRACTS
dc.subjectGAS-CHROMATOGRAPHY
dc.subjectANTIMALARIAL-DRUG
dc.subjectULTRAVIOLET DETECTION
dc.subjectMAJOR METABOLITE
dc.subjectANNUA L
dc.subjectPLASMA
dc.subjectDIHYDROARTEMISININ
dc.subjectARTEMETHER
dc.titleAnalysis of artemisinin by a packed-column supercritical fluid chromatography-atmospheric pressure chemical ionisation mass spectrometry technique
dc.typeArticle

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