Endoplasmic Reticulum Stress-Induced Apoptotic Effects of Novel 1-Pyrroline (3,4-Dihydro-2H-pyrrole) Derivatives on Breast Cancer Cells

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dc.contributor.authorAtmaca H.
dc.contributor.authorIlhan S.
dc.contributor.authorKorkmaz E.
dc.contributor.authorZora M.
dc.date.accessioned2024-07-22T08:04:11Z
dc.date.available2024-07-22T08:04:11Z
dc.date.issued2022
dc.description.abstractHeterocyclic compounds have emerged as promising and appealing scaffolds for developing effective antitumor agents. Here, the effects of synthesized 24 different 1-pyrroline derivatives (PDs) containing substituted aryl sulfide moiety were investigated on human breast cancer cell lines. The viability of cells was assessed via MTT assay. Reactive oxygen species (ROS) generation was analyzed via fluorescent dye CM-H2DCFDA. Apoptotic cells were determined via flow cytometry. Endoplasmic reticulum (ER) stress-associated protein levels were analyzed via western blot analysis. Four of the PDs (PD-12, -14, -16 and -17) had great cytotoxic selectivity against breast cancer cells. Apoptotic cell death was induced by PDs via the generation of ROS. PDs significantly increased the GRP78, p-PEAK, p-eIF2α, and CHOP protein levels indicating ER stress in breast cancer cells. These results imply that newly synthesized PDs may be potential anticancer agents as they selectively inhibit breast cancer cells. © 2022 Wiley-VHCA AG, Zurich, Switzerland.
dc.identifier.DOI-ID10.1002/cbdv.202200123
dc.identifier.issn16121872
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/12576
dc.language.isoEnglish
dc.publisherJohn Wiley and Sons Inc
dc.subject1 pyrroline(3,4 dihydro 2h pyrrole)derivative
dc.subjectantineoplastic agent
dc.subjectcisplatin
dc.subjectendoplasmic reticulum chaperone BiP
dc.subjectfluorescent dye
dc.subjectfluorouracil
dc.subjectgrowth arrest and DNA damage inducible protein 153
dc.subjectinitiation factor 2alpha
dc.subjectprotein Bax
dc.subjectpyrrole derivative
dc.subjectreactive oxygen metabolite
dc.subjectunclassified drug
dc.subjectantiapoptotic activity
dc.subjectArticle
dc.subjectbreast cancer cell line
dc.subjectcancer chemotherapy
dc.subjectcancer immunotherapy
dc.subjectcell proliferation
dc.subjectcell viability
dc.subjectcontrolled study
dc.subjectcytotoxicity
dc.subjectendoplasmic reticulum stress
dc.subjectfemale
dc.subjectflow cytometry
dc.subjectfolate metabolism
dc.subjectHep-G2 cell line
dc.subjecthuman
dc.subjecthuman cell
dc.subjectIC50
dc.subjectMCF-7 cell line
dc.subjectMTT assay
dc.subjectprotein expression level
dc.subjectquality of life
dc.subjectWestern blotting
dc.titleEndoplasmic Reticulum Stress-Induced Apoptotic Effects of Novel 1-Pyrroline (3,4-Dihydro-2H-pyrrole) Derivatives on Breast Cancer Cells
dc.typeArticle

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