Immunomodulatory Effects of MDA-MB-231-derived Exosome Mimetic Nanovesicles on CD4+ T Cell Line

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dc.contributor.authorOztatlici, M
dc.contributor.authorOzdemir, AT
dc.contributor.authorOztatlici, H
dc.contributor.authorKucukhuyuk, S
dc.contributor.authorOzdemir, RBO
dc.contributor.authorOrhan, H
dc.contributor.authorOzbilgin, K
dc.date.accessioned2024-07-18T12:05:58Z
dc.date.available2024-07-18T12:05:58Z
dc.description.abstractObjectives: The aim of this study is to investigate the immunomodulatory effects of MDA-MB-231 cells or MDA-MB231-derived exosome-mimetic nanovesicles (NVs) on CD4+ Jurkat cells. Methods: NVs were produced by the breakdown of MDA-MB-231 cells and the characterization of generated NVs were performed by using direct-ELISA and Flow cytometry methods. We co -cultured CD4+ Jurkat cells with MDA-MB-231 cells or MDA-MB-231-derived NVs for 48 h. Subsequently, expressions of pro -inflammatory and anti-inflammatory cytokines, and related transcription factors of CD4+ Jurkat cells were evaluated by qPCR method. Results: Clustering, which is the indicator of activation, was not seen in the CD4+ Jurkat cells co -cultured with MDAMB-231 cells. However, CD4+ Jurkat cell clusters were observed in the co -culture experiments with all NV concentrations. In addition, it was determined that the expressions of pro -inflammatory cytokines significantly increased while the expressions of anti-inflammatory cytokines was dramatically decreased in the NV -treated groups. On the contrary, opposite results were obtained in CD4+ Jurkat cells co -cultured with MDA-MB-231 cells. Moreover, TNF-alpha and Gata3 expressions were decreased in all groups. Conclusion: These preliminary findings from in -vitro experiments suggested that NVs could be a potential tool in cancer immunotherapy, but our data need to be supported by more comprehensive studies.
dc.identifier.other2587-196X
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/10104
dc.language.isoEnglish
dc.publisherKARE PUBL
dc.subjectBREAST-CANCER
dc.subjectSTEM-CELLS
dc.subjectTUMOR
dc.subjectTHERAPY
dc.titleImmunomodulatory Effects of MDA-MB-231-derived Exosome Mimetic Nanovesicles on CD4+ T Cell Line
dc.typeArticle

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