The effect of different implant biomaterials on the behavior of canine bone marrow stromal cells during their differentiation into osteoblasts

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dc.contributor.authorÖzdal-Kurt, F
dc.contributor.authorTuglu, I
dc.contributor.authorVatansever, HS
dc.contributor.authorTong, S
dc.contributor.authorSen, BH
dc.contributor.authorDeliloglu-Gürhan, SI
dc.date.accessioned2024-07-18T12:06:16Z
dc.date.available2024-07-18T12:06:16Z
dc.description.abstractWe investigated the effects of different implant biomaterials on cultured canine bone marrow stromal cells (BMSC) undergoing differentiation into osteoblasts (dBMSC). BMSC were isolated from canine humerus by marrow aspiration, cultured and differentiated on calcium phosphate scaffold (CPS), hydroxyapatite, hydroxyapatite in gel form and titanium mesh. We used the MTT method to determine the effects of osteogenic media on proliferation. The characteristics of dBMSC were assessed using alizarin red (AR), immunocytochemistry and osteoblastic markers including alkaline phosphatase/von Kossa (ALP/VK), osteocalcin (OC) and osteonectin (ON), and ELISA. The morphology of dBMSC on the biomaterials was investigated using inverted phase contrast microscopy and scanning electron microscopy. We detected expression of ALP/VK, AR, OC and ON by day 7 of culture; expression increased from day 14 until day 21. CPS supported the best adhesion, cell spreading, proliferation and differentiation of BMSCs. The effects of the biomaterials depended on their surface properties. Expression of osteoblastic markers showed that canine dBMSCs became functional osteoblasts. Tissue engineered stem cells can be useful clinically for autologous implants for treating bone wounds.
dc.identifier.issn1052-0295
dc.identifier.other1473-7760
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/10280
dc.language.isoEnglish
dc.publisherTAYLOR & FRANCIS LTD
dc.subjectMESENCHYMAL STEM-CELLS
dc.subjectIN-VITRO
dc.subjectTITANIUM
dc.subjectCULTURE
dc.subjectREPAIR
dc.subjectTISSUE
dc.subjectHYDROXYAPATITE
dc.subjectPHOSPHATE
dc.subjectSURFACE
dc.subjectGROWTH
dc.titleThe effect of different implant biomaterials on the behavior of canine bone marrow stromal cells during their differentiation into osteoblasts
dc.typeArticle

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