Molecular analyses of ADAMTS-1,-4,-5, and IL-17 a cytokine relationship in patients with ulcerative colitis

Simple item page
dc.contributor.authorBuran, T
dc.contributor.authorBatir, MB
dc.contributor.authorÇam, FS
dc.contributor.authorKasap, E
dc.contributor.authorÇöllü, F
dc.contributor.authorÇelebi, HBG
dc.contributor.authorSahin, M
dc.date.accessioned2024-07-18T12:00:54Z
dc.date.available2024-07-18T12:00:54Z
dc.description.abstractBackgroundUlcerative colitis (UC) is a chronic inflammatory bowel disease that develops due to the impaired immune response in genetically susceptible individuals, and its etiopathogenesis is not fully elucidated. IL-17 A is a cytokine that is produced by a type of immune cell called Th17 cells and is involved in the immune response and inflammation. On the other hand, ADAMTS-1, -4, and - 5 are enzymes that are involved in the breakdown of extracellular matrix proteins, including proteoglycans, which are important components of the intestinal wall. This study aimed to evaluate the relationship between interleukin 17 (IL-17 A) cytokine, which plays a role in the pathogenesis of ulcerative colitis, and the inflammation-controlled a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)-1, -4, and - 5 protein members.MethodsBowel tissue samples and blood serum from 51 patients with UC and 51 healthy controls were included in this study. mRNA expression levels of the ADAMTS-1, -4, -5, and IL-17 A were analyzed by RT-qPCR, and immunohistochemical analyses were performed to evaluate ADAMTS-1, -4, -5, and IL-17 A proteins in tissue samples. In addition, ELISA analysis determined serum levels of the ADAMTS-1, -4, -5, and IL-17 A.ResultsRT-qPCR results reveal that the expression of ADAMTS-1, -4, -5, and IL-17 A genes in the UC tissue samples were significantly high according to the control tissue samples. Also, ADAMTS-1, -4, -5, and IL-17 A proteins revealed enhanced expression pattern UC groups according to the control. Also, ADAMTS-1, -4, -5, and IL-17 A protein showed cytoplasmic localization patterns in both control and UC groups. The serum levels of ADAMTS-1,-5, and IL-17 A were significantly higher in UC samples than in the control group.ConclusionsWe observed a positive correlation between the ADAMTS-1, -5 and IL17A cytokine expression in UC samples. These results provide a new understanding of controlling crucial ADAMTS family protein members by IL-17 A cytokines with UC.
dc.identifier.other1471-230X
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/8055
dc.language.isoEnglish
dc.publisherBMC
dc.subjectINFLAMMATORY-BOWEL-DISEASE
dc.subjectGENETIC POLYMORPHISMS
dc.subjectEXPRESSION
dc.subjectINTERLEUKIN-17
dc.subjectSUSCEPTIBILITY
dc.subjectASSOCIATION
dc.subjectINSIGHTS
dc.subjectRECEPTOR
dc.subjectCELLS
dc.subjectTL1A
dc.titleMolecular analyses of ADAMTS-1,-4,-5, and IL-17 a cytokine relationship in patients with ulcerative colitis
dc.typeArticle

Files

Collections

Web of Science Koleksiyonu