Investigation of the toxicity of acetamiprid in SH-SY5Y neural\rcells

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dc.contributor.authorMustafa ÖZTATLICI
dc.contributor.authorHULYA OZTATLICI
dc.contributor.authorSuna Karadeniz Saygılı
dc.contributor.authorİlker Deniz Cingöz
dc.contributor.authorİsmail Kaya
dc.date.accessioned2025-04-14T05:52:33Z
dc.date.available2025-04-14T05:52:33Z
dc.date.issued2022
dc.description.abstractAim: Acetamiprid (ACE) is one of the most widely used neonicotinoids globally to protect\rcrops from insects. In this study aimed to investigate the potential neurotoxic activity of\racetamiprid on human neuroblastoma cell line SH-SY5Y cells.\rMaterials and Methods: MTT and Muse analysis were performed to examine the\reffect on cell viability. Increasing doses of ACE were administered for 24 hours in SHSY5\rneuroblastoma cell lines. NOS1, NOS2, NOS3; caspase-3 for assessment of apoptosis;\rKi67 immunocytochemical staining was performed to evaluate proliferation, and relative\rmRNA values of these markers were measured by qRT-PCR analysis method to evaluate\rthe efficacy of ACE on oxidative stress in neuroblastoma cell lines\rResults: The IC50 value for ACE 24 hours was found to be 21.35 mM. In SHSY5 cells,\rthe immunoreactivity of NOS1, NOS3, and caspase-3 markers in the ACE applied group\rincreased statistically significantly compared to the control group; Ki67 immunoreactivity\ralso decreased (p < 0.05). qRT-PCR results were consistent with immunocytochemical\rfindings, and relative mRNA values increased in ACE groups compared to the control\rgroup. Ki67 relative mRNA values decreased compared to the control group.\rConclusion: In our study, it was found that ACE suppressed proliferation in SH-SY5Y\rcells, induced apoptosis, and caused cell toxicity by increasing oxidative stress.
dc.identifier.DOI-ID10.5455/annalsmedres.2021.09.559
dc.identifier.urihttp://hdl.handle.net/20.500.14701/55389
dc.language.isoİngilizce
dc.subjectNörolojik Bilimler
dc.subjectToksikoloji
dc.subjectBiyokimya ve Moleküler Biyoloji
dc.titleInvestigation of the toxicity of acetamiprid in SH-SY5Y neural\rcells

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