The Neuroprotective Effect of Pioglitazone on NB2a Mouse Neuroblastoma Cell Culture

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dc.contributor.authorVural, K
dc.contributor.authorSeyrek, O
dc.date.accessioned2024-07-18T11:49:17Z
dc.date.available2024-07-18T11:49:17Z
dc.description.abstractPioglitazone (PGT) is a PPAR-gamma activator that has neuroprotective properties via different mechanisms. It is thought to be neuroprotective in both acute and chronic use. Chlorpyrifos (CPS) is an Organophosphate insecticide that leads to attention deficit and cognitive problems in children and its neurotoxic effects are well known. This study aims to investigate the neuroprotective effects of PGT on CPS neurotoxicity in NB2a cell in the culture medium. We investigated the cell viability and proliferation using MTT assay and the percentage of neurite inhibition was analysed by measuring neurite outgrowth. Apoptosis was evaluated using the apoptotic index in TUNEL staining. Cell proliferation was found to be significantly reduced by CPS (25 mu M), and this concentration-based reduction was prevented by PGT. Neurite outgrowth was inhibited by CPS (25 mu M), whereas PGT significantly reversed neurite inhibition at and above 10 mu M concentrations. The apoptotic index, which was increased using CPS (25 mu M), was observed to reduce using PGT, depending on the concentration. Organophosphate is harmful to human health, and to our knowledge, there is no treatment. In individuals exposed to chlorpyrifos toxicity, acute toxic effects on neurons may be prevented or treated by PGT.
dc.identifier.issn1300-6045
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/3897
dc.language.isoEnglish
dc.publisherKAFKAS UNIV, VETERINER FAKULTESI DERGISI
dc.subjectPPAR-GAMMA
dc.subjectINDUCED APOPTOSIS
dc.subjectDOWN-REGULATION
dc.subjectMPTP MODEL
dc.subjectIN-VITRO
dc.subjectACTIVATION
dc.subjectCHLORPYRIFOS
dc.subjectINHIBITION
dc.subjectAGONISTS
dc.subjectSH-SY5Y
dc.titleThe Neuroprotective Effect of Pioglitazone on NB2a Mouse Neuroblastoma Cell Culture
dc.typeArticle

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