Investigation of Bioactivity of Estragole Isolated from Basil Plant on Brain Cancer Cell Lines Using Nuclear Method

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dc.contributor.authorAvcibasi U.
dc.contributor.authorDewa M.T.
dc.contributor.authorKaratay K.B.
dc.contributor.authorKilcar A.Y.
dc.contributor.authorMuftuler F.Z.B.
dc.date.accessioned2025-04-10T11:03:57Z
dc.date.available2025-04-10T11:03:57Z
dc.date.issued2023
dc.description.abstractBackground: In recent years, there has been a significant increase in studies investigating the potential use of plant-origin products in the treatment and diagnosis of different types of cancer. Methods: In this study, Estragole (EST) was isolated from basil leaves via ethanolic extraction using an 80% ethanol concentration. The isolation process was performed using the High Performance Liquid Chromatography (HPLC) method. The EST isolated from the basil plant was radio-labeled with131I using the iodogen method. Quality control studies of the radiolabeled EST (131I-EST) were carried out by using Thin Layer Radio Chromatography (TLRC). Next, in vitro cell, culture studies were done to investigate the bio-affinity of plant-originated EST labeled with131I on human medulloblastoma (DAOY) and human glioblastoma-astrocytoma (U-87 MG) cell lines. Finally, the cytotoxicity of EST was determined, and cell uptake of131I-EST was investigated on cancer cell lines by incorporation studies. Results: As a result of these studies, it has been shown that131I-EST has a significant uptake on the brain cells. Conclusion: This result is very satisfying, and it has encouraged us to do in vivo studies for the molecule in the future. © 2023 Bentham Science Publishers.
dc.identifier.DOI-ID10.2174/1874471016666230110144021
dc.identifier.urihttp://hdl.handle.net/20.500.14701/45060
dc.publisherBentham Science Publishers
dc.titleInvestigation of Bioactivity of Estragole Isolated from Basil Plant on Brain Cancer Cell Lines Using Nuclear Method
dc.typeArticle

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