LC-MS/MS profiles and interrelationships between the enzyme inhibition activity, total phenolic content and antioxidant potential of Micromeria nervosa extracts
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Date
2020
Authors
Sarikurkcu C.
Andrade J.C.
Ozer M.S.
de Lima Silva J.M.F.
Ceylan O.
de Sousa E.O.
Coutinho H.D.M.
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Abstract
The objective of this study was to quantify the phenolic compounds and to evaluate and compare the biological activities of the ethyl acetate (EtOAc), methanolic (MeOH) and aqueous extracts from the Micromeria nervosa aerial parts, based on their antioxidant activity and enzymatic inhibition. Total phenolic and flavonoid contents were calculated and individual compo3unds were detected using LC-ESI-MS/MS. The antioxidant activity was determined using six different assays while enzymatic activity was determined by α-amylase and tyrosinase enzyme inhibition. The main phenolic constituents detected in the extracts were rosmarinic acid. In the antioxidant assays the aqueous extract was shown to be more efficient than the others. The EtOAc and MeOH extracts presented higher inhibitory activity with respect to α-amylase and tyrosinase. Regardless of the solvent, the results suggest M. nervosa aerial extracts present a biological potential due to their antioxidant activity and enzymatic inhibition. © 2020 Elsevier Ltd
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Keywords
alpha-Amylases, Antioxidants, Chromatography, High Pressure Liquid, Cinnamates, Depsides, Enzyme Inhibitors, Flavonoids, Lamiaceae, Monophenol Monooxygenase, Phenols, Plant Components, Aerial, Plant Extracts, Tandem Mass Spectrometry, Amylases, Antennas, Antioxidants, Enzyme activity, Plants (botany), acetic acid ethyl ester, amylase, antioxidant, monophenol monooxygenase, Nervosa micromeria extract, phenol derivative, plant extract, rosmarinic acid, unclassified drug, antioxidant, cinnamic acid derivative, depside, enzyme inhibitor, flavonoid, phenol derivative, plant extract, Anti-oxidant activities, Antioxidant potential, Biological potential, Enzymatic activities, Inhibition activity, Inhibitory activity, Phenolic compounds, Total phenolic content, aerial plant part, antioxidant activity, Article, controlled study, enzyme activity, enzyme inhibition, IC50, liquid chromatography-mass spectrometry, medicinal plant, Nervosa micromeria, chemistry, high performance liquid chromatography, Lamiaceae, metabolism, tandem mass spectrometry, Enzyme inhibition