Expression profiling of stem cell signaling alters with spheroid formation in CD133high/CD44high prostate cancer stem cells

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dc.contributor.authorOktem G.
dc.contributor.authorBilir A.
dc.contributor.authorUslu R.
dc.contributor.authorInan S.V.
dc.contributor.authorDemiray S.B.
dc.contributor.authorAtmaca H.
dc.contributor.authorAyla S.
dc.contributor.authorSercan O.
dc.contributor.authorUysal A.
dc.date.accessioned2025-04-10T11:13:22Z
dc.date.available2025-04-10T11:13:22Z
dc.date.issued2014
dc.description.abstractCancer stem cells (CSC) isolated from multiple tumor types differentiate in vivo and in vitro when cultured in serum; however, the factors responsible for their differentiation have not yet been identified. The first aim of the present study was to identify CD133high/CD44high DU145 prostate CSCs and compare their profiles with non-CSCs as bulk counterparts of the population. Subsequently, the two populations continued to be three-dimensional multicellular spheroids. Differentiation was then investigated with stem cell-related genomic characteristics. Polymerase chain reaction array analyses of cell cycle regulation, embryonic and mesenchymal cell lineage-related markers, and telomerase reverse transcriptase (TERT) and Notch signaling were performed. Immunohistochemistry of CD117, Notch1, Jagged1, Delta1, Sox2, c-Myc, Oct4, KLF4, CD90 and SSEA1 were determined in CSC and non-CSC monolayer and spheroid subcultures. Significant gene alterations were observed in the CD133high/CD44high population when cultured as a monolayer and continued as spheroid. In this group, marked gene upregulation was determined in collagen type 9 α1, Islet1 and cyclin D2. Jagged1, Delta-like 3 and Notch1 were respectively upregulated genes in the Notch signaling pathway. According to immunoreactivity, the staining density of Jagged1, Sox2, Oct4 and Klf-4 increased significantly in CSC spheroids. Isolated CSCs alter their cellular characterization over the course of time and exhibit a differentiation profile while maintaining their former surface antigens at a level of transcription or translation. The current study suggested that this differentiation process may be a mechanism responsible for the malignant process and tumor growth.
dc.identifier.DOI-ID10.3892/ol.2014.1992
dc.identifier.urihttp://hdl.handle.net/20.500.14701/49865
dc.publisherSpandidos Publications
dc.titleExpression profiling of stem cell signaling alters with spheroid formation in CD133high/CD44high prostate cancer stem cells
dc.typeArticle

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