The expression of forkhead transcription factors in decidua and placenta in patients with missed abortion
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Date
2015
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Abstract
Background: Forkhead transcription factors 3a (FOX03a) has pleiotropic biological functions in the female reproductive tract. FOX03a has a function in decidualization, in placental development, and also in inhibition of apoptosis. This study aims to investigate a possible role of FOX03a in missed abortion. Materials and Methods: Decidual and placental tissue samples were obtained from the women with unwanted pregnancy as the control group and with missed abortion as the patient group. Immunohistochemistry technique was utilized to compare FOX03a expression of the decidual cells in uterine decidual stroma and cytotrophoblast-syncytiotrophoblast cells in placental villous stroma. Immunohistochemistry was evaluated semi-quantitatively utilizing the H-score technique. Results: It was demonstrated that H-Scores of FOX03a expression in both uterine decidual stroma were increased in the missed abortion group (255.83±12.41) than in the normal pregnancy group (133.33±17.43). It was also shown that there was no difference between non-decidual area of the endometrium of the normal pregnancy and the missed abortion group (30.33±4.32; 39.66±14.30, respectively) and placental villous stroma (13.00±1.89; 13.00±1.67, respectively). However, the immunoreactivity of cytotrophoblast and syncytiotrophoblast cells significantly increased in the missed abortion group (18.83±1.47; 322.00±6.06, respectively) than in the normal pregnancy group (11.00±1.26; 254.00±8.17, respectively) (p < 0.05). Conclusion: These data support the hypothesis that increased FOX03a expression in missed abortion may prevent the discharge of dead fetus to maintain decidualization, prevention of oxidative stress, immunomodulation, and inhibition of apoptosis.
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Keywords
Abortion, Missed , Adult , Case-Control Studies , Decidua , Embryo Implantation , Female , Forkhead Transcription Factors , Humans , Immunohistochemistry , Placenta , Placentation , Pregnancy , Pregnancy Trimester, First , Young Adult , forkhead transcription factor , adult , apoptosis , Article , chorion villus , clinical article , control group , controlled study , cytotrophoblast , decidua , endometrium , female , fetus death , human , human tissue , immunohistochemistry , immunomodulation , immunoreactivity , missed abortion , oxidative stress , pathogenesis , placenta , protein expression , protein localization , stroma , syncytiotrophoblast , unwanted pregnancy , case control study , decidua , first trimester pregnancy , immunology , metabolism , missed abortion , nidation , placenta , pregnancy , reproduction , young adult