Chemoprotective effect of ascorbic acid, α-tocopherol, and selenium on cyclophosphamide-induced toxicity in the rat ovarium

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dc.contributor.authorGürgen S.G.
dc.contributor.authorErdoĝan D.
dc.contributor.authorElmas Ç.
dc.contributor.authorKaplanoĝlu G.T.
dc.contributor.authorÖzer Ç.
dc.date.accessioned2024-07-22T08:18:27Z
dc.date.available2024-07-22T08:18:27Z
dc.date.issued2013
dc.description.abstractObjective: The aim of the study was to evaluate the protective efficacy of ascorbic acid, α-tocopherol, and selenium by measuring the glutathione (GSH) levels and proliferating cell nuclear antigen (PCNA) and growth differentiation factor-9 (GDF-9) expression in the ovarian tissues of rats treated with cyclophosphamide (CP) therapy. Methods: Female Wistar rats were divided into 5 groups of 6 rats each: (I) control, (II) only CP, (III) CP + ascorbic acid, (IV) CP + α-tocopherol, and (V) CP + selenium. Immunohistochemical stainings and GSH protocol were then applied. Results: Following CP administration, the rats exhibited significantly lower GDF-9 expression in oocytes and PCNA expression in granulosa cells of follicles in all stages of development (P < 0.05). In CP + antioxidant groups (Groups III, IV, V), GDF-9 immunoreaction in oocytes and PCNA immunoreaction in granulosa cells of the developing follicles were found to show an increase towards the levels observed in the control group (P < 0.05). Conclusions: CP was found to cause remarkable degenerative effects in normal ovarian tissue, and we believe that this damage can be reduced and ovarian tissue can be spared from the toxic effects of CP by using antioxidants such as ascorbic acid, α-tocopherol, and selenium. © 2013 Elsevier Inc.
dc.identifier.DOI-ID10.1016/j.nut.2012.11.004
dc.identifier.issn18731244
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/17286
dc.language.isoEnglish
dc.subjectalpha-Tocopherol
dc.subjectAnimals
dc.subjectAntineoplastic Agents, Alkylating
dc.subjectAntioxidants
dc.subjectAscorbic Acid
dc.subjectCyclophosphamide
dc.subjectFemale
dc.subjectGlutathione
dc.subjectGranulosa Cells
dc.subjectGrowth Differentiation Factor 9
dc.subjectOocytes
dc.subjectOvarian Diseases
dc.subjectOvary
dc.subjectProliferating Cell Nuclear Antigen
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectSelenium
dc.subjectRattus
dc.subjectRattus norvegicus
dc.subjectalpha tocopherol
dc.subjectascorbic acid
dc.subjectcycline
dc.subjectcyclophosphamide
dc.subjectglutathione
dc.subjectgrowth differentiation factor 9
dc.subjectselenium
dc.subjectadverse outcome
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectantioxidant activity
dc.subjectarticle
dc.subjectcell population
dc.subjectcell protection
dc.subjectcontrolled study
dc.subjectdown regulation
dc.subjectdrug effect
dc.subjectdrug efficacy
dc.subjectfemale
dc.subjectgranulosa cell
dc.subjectimmunofluorescence test
dc.subjectimmunohistochemistry
dc.subjectnonhuman
dc.subjectoocyte
dc.subjectorgan weight
dc.subjectovary disease
dc.subjectovary follicle cell
dc.subjectovary follicle development
dc.subjectovary toxicity
dc.subjectovary weight
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectrat
dc.subjectsignal transduction
dc.subjecttissue degeneration
dc.titleChemoprotective effect of ascorbic acid, α-tocopherol, and selenium on cyclophosphamide-induced toxicity in the rat ovarium
dc.typeArticle

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