The cytotoxic and antiproliferative effect of Polygala saponin XLIV on the human colorectal carcinoma cell line

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dc.contributor.authorBecer E.
dc.contributor.authorHanoğlu A.
dc.contributor.authorÜnlü A.
dc.contributor.authorAydın Z.U.
dc.contributor.authorDönmez A.A.
dc.contributor.authorJurt S.
dc.contributor.authorVatansever S.H.
dc.contributor.authorÇalış İ.
dc.date.accessioned2025-04-10T11:01:46Z
dc.date.available2025-04-10T11:01:46Z
dc.date.issued2025
dc.description.abstractObjectives: Saponins are secondary metabolites naturally found in plants with diverse pharmacological properties such as anticancer. This research aimed to explore the anti-cancer properties of Polygalasaponin XLIV (PS-XLIV) in a human colorectal carcinoma cell line derived from Polygala vulgaris roots. Methods: HCT166 cells were treated with different PS-XLIV concentrations and incubated for 24 and 48 h. We used immunocytochemistry to investigate PS-XLIV’s anti-cancer properties, employing antibodies targeting WNT3A, WNT11, STAT3, β-catenin, and Ki-67. The IC50 value of PS-XLIV was 80 μg/mL in HCT116 cells. WNT11, STAT3, β-catenin, and Ki-67. Immunoreactivities significantly decreased in PS-XLIV-treated HCT116 cells than in control group cells. Results: After PS-XLIV treatment, the epithelial morphology of cells was protected; however, the number of cells was less than that of the control group cells. While WNT3A immunoreactivity was similar in both groups, WNT11 and β-catenin immunoreactivities were decreased after PS-XLIV application. In addition, the PS-XLIV treated group exhibited significantly weaker Ki-67 immunoreactivity, STAT3 immunoreactivty was moderated after PS-XLIV application. Conclusions: For the first time, the anticancer effects of PS-XLIV isolated from P. vulgaris on HCT116 cells were shown. The anticancer effect may involve PS-XLIV reducing WNT11, β-catenin, STAT3, and Ki-67 activation pathways in HCT116 cells. © 2024 the author(s)
dc.identifier.DOI-ID10.1515/tjb-2024-0215
dc.identifier.urihttp://hdl.handle.net/20.500.14701/43626
dc.publisherWalter de Gruyter GmbH
dc.titleThe cytotoxic and antiproliferative effect of Polygala saponin XLIV on the human colorectal carcinoma cell line
dc.typeArticle

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