Evaluation Of VEGF, EGF-R AND TGF-α In Placenta Of Pregnancies With Preeclampsia By Immunohistochemistry
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Objective: The aim of the study is to determine VEGF, EGFR and TGF-alpha with the immunohistochemical staining in placenta biopsies of patients with preeclampsia. Design: Prospective case control study. Materials and Methods: Biopsies from the placenta were obtained from ten patients with preeclapmsia and ten patients of control group during the cesarean section. Placenta biopsies were examined for VEGF, EGF-R and TGF-alpha distribution with avidin-biotinperoxidase immunohistochemistry. The staining intensities were graded semi-quantitatively by the two histologists who were blinded to the groups and the H-score was calculated by light microscopic examination. Results: The VEGF, EGFR and TGF-alpha expression was significantly higher in placenta biopsies of preeclamptic patient compared to controls (271.2 +/- 22.65 versus 201.9 +/- 12.33, p=0.000; 186.3 +/- 4.98 versus 150.3 +/- 5.7, p=0.000; 185.1 +/- 7.48 versus 169.2 +/- 6.19, p=0.000; respectively). Conclusion: Immunostaining of VEGF, EGF-R and TGF-alpha is significantly higher in placenta biopsies in patients with preeclampsia.