Evaluation of the effects of gonadotropin-relasing hormone antagonist (GnRH-ant) and agonist (GnRH-a) in the prevention of postoperative adhesion formation in a rat model with immunohistochemical analysis

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dc.contributor.authorTamay, AG
dc.contributor.authorGuvenal, T
dc.contributor.authorMicili, SC
dc.contributor.authorYildirim, Y
dc.contributor.authorOzogul, C
dc.contributor.authorKoyuncu, FM
dc.contributor.authorKoltan, SO
dc.date.accessioned2024-07-18T12:03:52Z
dc.date.available2024-07-18T12:03:52Z
dc.description.abstractObjective: To investigate the effects of GnRH antagonist (GnRH-ant) and agonist (GnRH-a) in the prevention of postoperative pelvic adhesions by a visual scoring system and immunohistochemical methods in a rat uterine horn model. Design: Controlled experimental animal study. Setting: Animal laboratory at an academic research environment. Animal(s): Twenty-one Wistar albino rats. Intervention(s): Rats were randomized into three groups. One week before the operation the rats received either GnRH-ant or GnRH-a or saline solution; they then underwent surgical laparotomy, and both uterine horns were traumatized by a scalpel. Three weeks later, all rats were sacrificed and extension and severity of the adhesions in each group were scored by a visual scoring system. Adhesion tissues were evaluated immunohistochemically for vitronectin and u-PAR. Main Outcome Measure(s): Scores of extend and severity of adhesions and staining of vitronectin and u-PAR. Result(s): The extent of adhesion scores were 1.85 +/- 0.86, 0.78 +/- 1.05, and 0.42 +/- 0.64, and the severity of adhesion scores were 1.71 +/- 0.91, 0.57 +/- 0.85, 0.50 +/- 0.75 for control, GnRH-ant, and GnRH-a groups, respectively. The extent and severity of adhesions were significantly lower in both GnRH-ant and GnRH-a groups when compared with the control group. Adhesion extent scores in the GnRH-a group were lower than in the GnRHant group, but this difference was not significant. vitronectin and u-PAR staining were significantly greater in both the GnRH-ant and GnRH-a groups than in the control group. Conclusion(s): GnRH-ant as well as GnRH-a reduced postoperative adhesion formation in a rat model. This finding was supported immunohistochemically by vitronectin and u-PAR staining. (Fertil Steril (R) 2011; 96: 1230-3. (C) 2011 by American Society for Reproductive Medicine.)
dc.identifier.issn0015-0282
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/9414
dc.language.isoEnglish
dc.publisherELSEVIER SCIENCE INC
dc.subjectUTERINE HORN MODEL
dc.subjectRINGERS LACTATE
dc.subjectGROWTH-FACTOR
dc.subjectRABBIT MODEL
dc.subjectENDOMETRIOSIS
dc.subjectBARRIERS
dc.subjectTHERAPY
dc.subjectPATHOPHYSIOLOGY
dc.subjectVITRONECTIN
dc.subjectPERITONEUM
dc.titleEvaluation of the effects of gonadotropin-relasing hormone antagonist (GnRH-ant) and agonist (GnRH-a) in the prevention of postoperative adhesion formation in a rat model with immunohistochemical analysis
dc.typeArticle; Proceedings Paper

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