Clinicopathological characteristics and mutation profile of BRAF and NRAS mutation in cutaneous melanomas in the Western Turkish population
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Date
2018
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Abstract
Background/aim: Malignant melanoma is the most common cause of death due to skin cancers. The most common mutations in RAFRAS pathway from tumor oncogenes are BRAF and NRAS. In this study, we analyzed the frequency of BRAF and NRAS gene mutations and investigated their association with clinicopathological features of melanomas in the Turkish population. Materials and methods: 65 primary cutaneous melanoma were included in the study. The mutations were evaluated with real-time PCR-based PCR-array through allele-specific amplification, and the results were correlated with various clinicopathological characteristics. Results: 52.3% of the patients were female and 47.7% were male. The mean age of the patients with a mutation was lower than those without mutation. 16 patients had BRAF mutation. 12 patients had NRAS mutation. NRAS mutation was statistically more common in men (P = 0.036). The number of mitoses increased with the increase of the tumor thickness (P = 0.003). There was more mitosis in the presence of ulceration (P = 0.05). A total of 41.7% of NRAS mutations had adjuvant chemotherapy. Conclusion: We found lower mutation rate when compared to regional studies. NRAS mutation was common in men. This is the first study from our region evaluating the prognostic value of clinical stage and necessity of adjuvant treatment with the presence of BRAF and NRAS mutations. © TÜBİTAK.
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Keywords
DNA Mutational Analysis , Female , GTP Phosphohydrolases , Humans , Male , Melanoma , Membrane Proteins , Middle Aged , Mutation , Proto-Oncogene Proteins B-raf , Real-Time Polymerase Chain Reaction , Skin Neoplasms , Turkey , B Raf kinase , B Raf kinase , BRAF protein, human , guanosine triphosphatase , membrane protein , NRAS protein, human , adult , aged , analysis of variance , Article , cancer adjuvant therapy , controlled study , correlation analysis , cutaneous melanoma , disease free survival , DNA extraction , DNA microarray , evaluation and follow up , exon , female , gene , gene amplification , gene mutation , genetic association , growth curve , head and neck carcinoma , histology , human , limb tumor , major clinical study , male , malignant lentigo , melanoma , middle aged , mitosis , NRAS gene , oncogene , overall survival , pathological anatomy , prognostic assessment , real time polymerase chain reaction , signal transduction , skin ulcer , trunk , tumor associated leukocyte , tumor localization , tumor volume , Turkish citizen , dna mutational analysis , genetics , melanoma , mutation , skin tumor , turkey (bird)