Clinicopathological characteristics and mutation profile of BRAF and NRAS mutation in cutaneous melanomas in the Western Turkish population

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dc.contributor.authorEvrenos M.K.
dc.contributor.authorTemiz P.
dc.contributor.authorÇam F.S.
dc.contributor.authorYaman M.
dc.contributor.authorYoleri L.
dc.contributor.authorErmertcan A.T.
dc.date.accessioned2024-07-22T08:10:08Z
dc.date.available2024-07-22T08:10:08Z
dc.date.issued2018
dc.description.abstractBackground/aim: Malignant melanoma is the most common cause of death due to skin cancers. The most common mutations in RAFRAS pathway from tumor oncogenes are BRAF and NRAS. In this study, we analyzed the frequency of BRAF and NRAS gene mutations and investigated their association with clinicopathological features of melanomas in the Turkish population. Materials and methods: 65 primary cutaneous melanoma were included in the study. The mutations were evaluated with real-time PCR-based PCR-array through allele-specific amplification, and the results were correlated with various clinicopathological characteristics. Results: 52.3% of the patients were female and 47.7% were male. The mean age of the patients with a mutation was lower than those without mutation. 16 patients had BRAF mutation. 12 patients had NRAS mutation. NRAS mutation was statistically more common in men (P = 0.036). The number of mitoses increased with the increase of the tumor thickness (P = 0.003). There was more mitosis in the presence of ulceration (P = 0.05). A total of 41.7% of NRAS mutations had adjuvant chemotherapy. Conclusion: We found lower mutation rate when compared to regional studies. NRAS mutation was common in men. This is the first study from our region evaluating the prognostic value of clinical stage and necessity of adjuvant treatment with the presence of BRAF and NRAS mutations. © TÜBİTAK.
dc.identifier.DOI-ID10.3906/sag-1802-80
dc.identifier.issn13000144
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15075
dc.language.isoEnglish
dc.publisherTurkiye Klinikleri Journal of Medical Sciences
dc.rightsAll Open Access; Bronze Open Access
dc.subjectDNA Mutational Analysis
dc.subjectFemale
dc.subjectGTP Phosphohydrolases
dc.subjectHumans
dc.subjectMale
dc.subjectMelanoma
dc.subjectMembrane Proteins
dc.subjectMiddle Aged
dc.subjectMutation
dc.subjectProto-Oncogene Proteins B-raf
dc.subjectReal-Time Polymerase Chain Reaction
dc.subjectSkin Neoplasms
dc.subjectTurkey
dc.subjectB Raf kinase
dc.subjectB Raf kinase
dc.subjectBRAF protein, human
dc.subjectguanosine triphosphatase
dc.subjectmembrane protein
dc.subjectNRAS protein, human
dc.subjectadult
dc.subjectaged
dc.subjectanalysis of variance
dc.subjectArticle
dc.subjectcancer adjuvant therapy
dc.subjectcontrolled study
dc.subjectcorrelation analysis
dc.subjectcutaneous melanoma
dc.subjectdisease free survival
dc.subjectDNA extraction
dc.subjectDNA microarray
dc.subjectevaluation and follow up
dc.subjectexon
dc.subjectfemale
dc.subjectgene
dc.subjectgene amplification
dc.subjectgene mutation
dc.subjectgenetic association
dc.subjectgrowth curve
dc.subjecthead and neck carcinoma
dc.subjecthistology
dc.subjecthuman
dc.subjectlimb tumor
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectmalignant lentigo
dc.subjectmelanoma
dc.subjectmiddle aged
dc.subjectmitosis
dc.subjectNRAS gene
dc.subjectoncogene
dc.subjectoverall survival
dc.subjectpathological anatomy
dc.subjectprognostic assessment
dc.subjectreal time polymerase chain reaction
dc.subjectsignal transduction
dc.subjectskin ulcer
dc.subjecttrunk
dc.subjecttumor associated leukocyte
dc.subjecttumor localization
dc.subjecttumor volume
dc.subjectTurkish citizen
dc.subjectdna mutational analysis
dc.subjectgenetics
dc.subjectmelanoma
dc.subjectmutation
dc.subjectskin tumor
dc.subjectturkey (bird)
dc.titleClinicopathological characteristics and mutation profile of BRAF and NRAS mutation in cutaneous melanomas in the Western Turkish population
dc.typeArticle

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