Effect of neocuproine, a selective Cu(I) chelator, on nitrergic relaxations in the mouse corpus cavernosum
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Date
2000
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Abstract
The effects of neocuproine and bathocuproine, Cu(I) and Cu(II) chelators, respectively, were studied on relaxations in response to electrical field stimulation, acetylcholine, S-nitrosoglutathione, acidified sodium nitrite and sodium nitroprusside in the mouse corpus cavernosum precontracted with phenylephrine. Neocuproine significantly inhibited relaxations induced by electrical field stimulation, acetylcholine and S- nitrosoglutathione, but not by acidified sodium nitrite and sodium nitroprusside. The pre-prepared neocuproine-Cu(I) complex was ineffective on the responses. The discrepancy between the shape of relaxations in response to electrical field stimulation or to acetylcholine and S-nitrosoglutathione was abolished by adding CuCl2 into the bathing medium. The copper action was blocked by neocuproine but not by bathocuproine. However, the pre-prepared bathocuproine-Cu(II) complex did not accelerate the relaxations affected by CuCl2. These findings suggest that a Cu(I)-dependent mechanism may play a role in the relaxation induced by the endogenous relaxant factor as well as by S-nitrosoglutathione in mouse cavernosal tissue. (C) 2000 Elsevier Science B.V.
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Acetylcholine , Animal , Chelating Agents , Copper , Dose-Response Relationship, Drug , Electric Stimulation , Glutathione , Male , Mice , Nitric Oxide , Nitroprusside , Nitroso Compounds , Penis , Phenanthrolines , Phenylephrine , S-Nitrosoglutathione , Support, Non-U.S. Gov't , 2,9 dimethyl 1,10 phenanthroline , acetylcholine , chelating agent , copper chloride , copper ion , nitroprusside sodium , phenylephrine , s nitrosoglutathione , sodium nitrite , animal tissue , article , chelation , controlled study , corpus cavernosum , electrostimulation , male , mouse , nonhuman , priority journal , signal transduction , smooth muscle relaxation