Combined gossypol and zoledronic acid treatment results in synergistic induction of cell death and regulates angiogenic molecules in ovarian cancer cells

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dc.contributor.authorAtmaca H.
dc.contributor.authorGorumlu G.
dc.contributor.authorKaraca B.
dc.contributor.authorDegirmenci M.
dc.contributor.authorTunali D.
dc.contributor.authorCirak Y.
dc.contributor.authorPurcu D.U.
dc.contributor.authorUzunoglu S.
dc.contributor.authorKarabulut B.
dc.contributor.authorSanli U.A.
dc.contributor.authorUslu R.
dc.date.accessioned2025-04-10T11:15:52Z
dc.date.available2025-04-10T11:15:52Z
dc.date.issued2009
dc.description.abstractIn the present study, we aimed to evaluate the possible synergistic, cytotoxic effects of combination treatment of gossypol and zoledronic acid, in human ovarian cancer cell lines, OVCAR-3 and MDAH-2774, and to elucidate the role of this novel combination treatment on angiogenesis-related molecules in ovarian cancer. The XTT cell viability assay was used for showing cytotoxicity. Both DNA fragmentation by ELISA assay and caspase 3/7 activity measurement were used for demonstrating apoptosis. To elucidate the angiogenic molecules affected by combination treatment, mRNA levels of angiogenic molecules were measured using the Human Angiogenesis RT2 Profiler™ PCR Array (SuperArray, Frederick, MD) in ovarian cancer cell lines, OVCAR-3 and MDAH-2774.The combined treatment resulted in significant, synergistic cytotoxicity, and induced apoptosis. This effect was observed to happen in a dose- and time-dependent manner. Moreover, the combination treatment of 10 μM gossypol and 5 μM zoledronic acid resulted in significant down-regulation (≥ thee-fold) in mRNA levels of some pivotal angiogenic molecules in OVCAR-3 and MDAH-2774 cells as compared to the untreated control. However, this effect was different in the two ovarian cancer cell lines observed. Gossypol, in combination with zoledronic acid, may provide a rational treatment option for ovarian cancer, not only by direct inhibition of cell proliferation, but also inhibition of angiogenesis-related molecules.
dc.identifier.DOI-ID10.1684/ecn.2009.0159
dc.identifier.urihttp://hdl.handle.net/20.500.14701/51348
dc.titleCombined gossypol and zoledronic acid treatment results in synergistic induction of cell death and regulates angiogenic molecules in ovarian cancer cells
dc.typeArticle

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