IMMUNE RESPONSES ELICITED BY THE RECOMBINANT ERP, HSPR, LPPX, MMAA4, AND OMPA PROTEINS FROM MYCOBACTERIUM TUBERCULOSIS IN MICE

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dc.contributor.authorOkay, S
dc.contributor.authorÇetin, R
dc.contributor.authorKarabulut, F
dc.contributor.authorDogan, C
dc.contributor.authorSürücüoglu, S
dc.contributor.authorKizildogan, AK
dc.date.accessioned2024-07-18T12:03:33Z
dc.date.available2024-07-18T12:03:33Z
dc.description.abstractImmunogenic potency of the recombinant Erp, HspR, LppX, MmaA4, and OmpA proteins from Mycobacterium tuberculosis (MTB), formulated with Montanide ISA 720 VG adjuvant, was evaluated in BALB/cmice for the first time in this study. The five vaccine formulations, adjuvant, and BCG vaccine were subcutaneously injected into mice, and the sera were collected at days 0, 15, 30, 41, and 66. The humoral and cellular immune responses against vaccine formulations were determined by measuring serum IgG and serum interferon-gamma (IFN-gamma) and interleukin-12 (IL-12) levels, respectively. All formulations significantly increased IgG levels post-vaccination. The highest increase in IFN-gamma level was provided by MmaA4 formulation. The Erp, HspR, and LppX formulations were as effective as BCG in enhancement of IFN-gamma level. The most efficient vaccine boosting the IL-12 level was HspR formulation, especially at day 66. Erp formulation also increased the IL-12 level more than BCG at days 15 and 30. The IL-12 level boosted by MmaA4 formulation was found to be similar to that by BCG. OmpA formulation was inefficient in enhancement of cellular immune responses. This study showed that MmaA4, HspR, and Erp proteins from MTB are successful in eliciting both humoral and cellular immune responses in mice.
dc.identifier.issn1217-8950
dc.identifier.other1588-2640
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/9175
dc.language.isoEnglish
dc.publisherAKADEMIAI KIADO ZRT
dc.subjectGUINEA-PIG MODEL
dc.subjectPROTECTIVE EFFICACY
dc.subjectDNA VACCINE
dc.subjectANTIGEN
dc.subjectIMMUNOGENICITY
dc.subjectSURFACE
dc.subjectBCG
dc.subjectCHALLENGE
dc.subjectINFECTION
dc.subjectSURVIVAL
dc.titleIMMUNE RESPONSES ELICITED BY THE RECOMBINANT ERP, HSPR, LPPX, MMAA4, AND OMPA PROTEINS FROM MYCOBACTERIUM TUBERCULOSIS IN MICE
dc.typeArticle

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