An Experimental Study of Radiation Effect on Normal Tissue: Analysis of HIF-1α, VEGF, eIF2, TIA-1, and TSP-1 Expression

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dc.contributor.authorAktas, C
dc.contributor.authorKurtman, C
dc.contributor.authorÖzbilgin, MK
dc.contributor.authorTek, I
dc.contributor.authorToprak, SK
dc.date.accessioned2024-07-18T12:06:26Z
dc.date.available2024-07-18T12:06:26Z
dc.description.abstractObjective: This study investigated whether or not the stress and hypoxia, which are the effects of radiation on normal vascular endothelium, leading to the release of HIF-1 alpha, VEGF, eIF2, TIA-1, and TSP-1 were related and the possibility of them stimulating angiogenesis. Materials and Methods: Twenty-four male Swiss Albino mice were separated into 4 groups. The first group was the control group (Group 1), and the second, third, and fourth groups were euthanized after 24 h (Group 2), 48 h (Group 3), and 7 days (Group 4), respectively. A single-fractioned 10 Gy of ionizing radiation was applied to all mice's pelvic zone with Co-60. Bladders were removed completely from the pelvic region. Immunohistochemistry and light microscopy were used to investigate whether there would be an increase or not in the angiogenesis pathway by using the HIF-1 alpha, VEGF, eIF2, TIA-1, and TSP-1 antibodies. Results: The HIP-1 alpha antibody showed strong staining in Group 3, while the staining intensity was less in other groups. VEGF showed weak staining in Groups 1 and 4, while moderate staining in Group 2 and strong staining in Group 3 was observed. eIF2 showed strong staining in Groups 1 and 4. Groups 2 and 3 were stained weakly. In the present study, staining with TSP-1 was very strong in the samples belonging to Group 1, while other groups showed very weak staining. Conclusion: When normal tissue was exposed to radiation, the positively effective factors (HIF-1, VEGF, eIF2, and TIA-1) on the angiogenesis pathway were increased while the negative factor (TSP-1) was decreased. Radiation may initiate physiological angiogenesis in the normal tissue and accelerate healing in the damaged normal tissue.
dc.identifier.issn1300-7777
dc.identifier.other1308-5263
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/10373
dc.language.isoEnglish
dc.publisherGALENOS YAYINCILIK
dc.subjectIONIZING-RADIATION
dc.subjectSTRESS GRANULES
dc.subjectTUMOR-GROWTH
dc.subjectHYPOXIA
dc.subjectINTERLEUKIN-8
dc.subjectANGIOGENESIS
dc.subjectRADIOTHERAPY
dc.subjectACTIVATION
dc.subjectAPOPTOSIS
dc.subjectPATHWAYS
dc.titleAn Experimental Study of Radiation Effect on Normal Tissue: Analysis of HIF-1α, VEGF, eIF2, TIA-1, and TSP-1 Expression
dc.typeArticle

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