Simultaneous determination of six antibiotics belonging to four different classes in chicken meat BY HPLC/DAD and verification BY LC-MS/MS
No Thumbnail Available
Date
2023
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The hypothesis of this study was to develop an extraction method allowing side-by-side extraction of six antibiotics belonging to four different classes and an HPLC/DAD method for the determination of their residues in chicken breast meat. The validation data showed that this hypothesis was achieved. The results were then confirmed by LC-MS/MS method. Sample preparation was based on the classical solid–liquid extraction with methanolic citric acid. The average recoveries were satisfactory and ranged from 75.68 to 101.3%. The linearity of the developed HPLC/DAD method was very high in the concentration range studied (R2 > 0.9969). The accuracy and precision of the analytical method were between –10.8 and 12.0% relative error and 0.82 to 10.1% relative standard deviation, respectively. The LODs for five antibiotics ranged from 0.6 to 2.7 µg kg−1 and LOQs ranged from 2.0 to 8.9 µg kg−1. For penicillin G, the LOD was 0.16 and LOQ was 0.52 mg kg−1. © 2023 Elsevier Ltd
Description
Keywords
Animals , Anti-Bacterial Agents , Chickens , Chromatography, High Pressure Liquid , Chromatography, Liquid , Meat , Tandem Mass Spectrometry , Animals , Extraction , Meats , antibiotic agent , chloramphenicol , chlortetracycline , citric acid , enrofloxacin , food additive , oxytetracycline , penicillin G , tetracycline , antiinfective agent , Chicken meat , Chloramphenicol , Different class , Enrofloxacin , Extraction method , Hplc/dad , LC-MS-MS , Penicillin G , Simultaneous determinations , Tetracycline , accuracy , analytic method , analytical parameters , animal experiment , animal food , Article , breast , chemical structure , chicken meat , chromatography , concentration (parameter) , controlled study , extraction , Gallus gallus , high performance liquid chromatography , hypothesis , limit of detection , limit of quantitation , liquid chromatography-mass spectrometry , mass spectrometry , matrix effect , nonhuman , poultry farming , retention time , temperature , toxicity , animal , Gallus gallus , high performance liquid chromatography , liquid chromatography , meat , procedures , tandem mass spectrometry , Antibiotics