The effect of resveratrol on the histologic characteristics of the cochlea in diabetic rats
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2019
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Abstract
Objectives/Hypothesis: The aim of this study was to investigate changes in the cochlea and the potential dose-dependent effects of resveratrol (RSV) against diabetes mellitus (DM) ototoxicity. Study Design: Animal model. Methods: Twenty-four male Wistar albino rats were divided into four groups. Baseline distortion product otoacoustic emission (DPOAE) measurements were evaluated. Group I was the control group, group II was made diabetic with single-dose streptozotocin, and groups III and IV were rendered diabetic as group II and administered 10 and 20 mg RSV, respectively, intraperitoneally for 4 weeks. All animals were sacrificed after repeated DPOAE measurement. Apoptosis was investigated using caspase-3, Bax (Bcl-associated X protein), and TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) staining. Results: The DPOAE values in the diabetic group were found to be significantly lower compared with the other groups at 5,714 Hz and 8,000 Hz (P <.05). No significant difference in otoacoustic emission was detected in the comparison of the RSV doses (P >.05). The histopathologic investigation using caspase-3, Bax, and TUNEL staining showed that the mean rank of the diabetic group was significantly higher compared with the RSV10, RSV20, and control groups (DM > RSV10 > RSV20 > control) (P <.05). Conclusions: These results imply that RSV administration offered statistically significant protection for the cochleas of rats against diabetes. This protective effect improved histologically with higher doses. Level of Evidence: NA Laryngoscope, 129:E1–E6, 2019. © 2018 The American Laryngological, Rhinological and Otological Society, Inc.
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Animals , Apoptosis , Cochlea , Diabetes Mellitus, Experimental , Disease Models, Animal , Dose-Response Relationship, Drug , Immunohistochemistry , In Situ Nick-End Labeling , Male , Otoacoustic Emissions, Spontaneous , Rats , Rats, Wistar , Resveratrol , caspase 3 , protein Bax , resveratrol , resveratrol , animal experiment , animal model , Article , cochlea , controlled study , Corti organ , distortion product otoacoustic emission , histopathology , immunohistochemistry , male , nonhuman , ototoxicity , priority journal , rat , signal noise ratio , streptozotocin-induced diabetes mellitus , TUNEL assay , animal , apoptosis , cochlea , disease model , dose response , drug effect , experimental diabetes mellitus , pathology , spontaneous otoacoustic emission , Wistar rat