Isolation and in vitro cultivation of human urine-derived cells: an alternative stem cell source

Simple item page
dc.contributor.authorSeçil Erden TAYHAN
dc.contributor.authorGönül Tezcan KELEŞ
dc.contributor.authorİsmet TOPÇU
dc.contributor.authorErol MİR
dc.contributor.authorSaime İsmet DELİLOĞLU GÜRHAN
dc.date.accessioned2024-07-24T09:09:14Z
dc.date.available2024-07-24T09:09:14Z
dc.date.issued2017
dc.description.abstractObjective: For in vitro tissue engineering in urology, stem cells are commonly isolated from tissue specimensobtained during open or endoscopic surgery. Within the context of the present study our aim was toisolate cells from human urine by an alternative and safe technique rather than using the indicated method.Material and methods: After human urine samples had been collected from young and healthy donorsvia urethral catheterization, cells were precipitated by centrifugation and cultured. Following this isolationprocedure, cells were characterized by immunocytochemical method using specific antibodies.Results: When these cells were characterized by immunocytochemical methods using specific antibodiessome of them were positive for mesenchymal stem cell marker CD90 while the others were labelled withurothelial marker cytokeratin 7. When all these results were taken into consideration, urothelial cells togetherwith stem cells were observed in the urine- derived cell population.Conclusion: According to the results obtained from this study human urine may be preferred as an alternativestem cell and urothelial cell source in that this method is and easily reproducible non-invasive method
dc.identifier.DOI-ID10.5152/tud.2017.93797
dc.identifier.issn2149-3235
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/21930
dc.language.isoeng
dc.subject[Fen > Tıp > Üroloji ve Nefroloji]
dc.titleIsolation and in vitro cultivation of human urine-derived cells: an alternative stem cell source
dc.typeAraştırma Makalesi

Files

Collections

TR-Dizin Koleksiyonu