Effects of leptin on the viability of human ovarian cancer cells and changes in cytokine expression levels
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Date
2023
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Abstract
Background: Obesity is associated with increased mortality among ovarian cancer and is a poor prognostic factor. There are significant links between the leptin hormone, a product of the obesity gene, and the development of ovarian cancer. Leptin is a vital hormone-like cytokine secreted from adipose tissue and is mainly involved in the maintenance of energy homeostasis. It regulates several intracellular signaling pathways and also interacts with various hormones and energy regulators. It acts as a growth factor by stimulating cell proliferation and differentiation and in this way contributes to cancer cell development. The aim of the study was to investigate the effects of leptin on human ovarian cancer cells. Methods: In this study, the effects of increasing the concentration of leptin were investigated on the cell viability of OVCAR-3 and MDAH-2774 ovarian cancer lines by MTT assay. Moreover, to elucidate the molecular mechanisms of leptin in ovarian cancer cells, changes in the expression levels of 80 cytokines were evaluated after leptin treatment via a human cytokine antibody array. Results: Leptin increases the proliferation of both ovarian cancer cell lines. IL-1 level was increased in OVCAR-3 cells and TGF-β level was increased in MDAH-2774 cells after leptin treatment. A decrease in IL-2, MCP-2/CCL8 and MCP-3/CCL7 levels was detected in both ovarian cancer cell lines with leptin administration. An increase in IL-3 and IL-10 expressions, insulin-like growth factor binding proteins (IGFBP) IGFBP-1, IGFBP-2 and IGFBP-3 levels were detected in both ovarian cancer cell lines with leptin administration. In conclusion; leptin has a proliferative effect on human ovarian cancer cell lines and affects different cytokines in different types of ovarian cancer cells. Copyright 2023 Dincer et al.
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cytokine , epidermal growth factor , fibroblast growth factor 6 , interleukin 1 , interleukin 10 , interleukin 12 , interleukin 13 , interleukin 16 , interleukin 2 , interleukin 3 , interleukin 5 , interleukin 8 , leptin , monocyte chemotactic protein 2 , monocyte chemotactic protein 3 , placental growth factor , platelet derived growth factor BB , somatomedin binding protein 1 , somatomedin binding protein 2 , somatomedin binding protein 3 , transforming growth factor beta , Article , cell proliferation , cell viability , cell viability assay , chemoluminescence , controlled study , female , human , human cell , MDAH 2774 cell line , MTT assay , obesity , ovarian cancer cell line , ovary cancer , OVCAR-3 cell line , XTT assay