Effects of leptin on the viability of human ovarian cancer cells and changes in cytokine expression levels

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dc.contributor.authorDincer F.
dc.contributor.authorAtmaca H.
dc.contributor.authorAkman L.
dc.contributor.authorOktay L.M.
dc.contributor.authorKaraca B.
dc.contributor.authorTerek M.C.
dc.date.accessioned2024-07-22T08:03:35Z
dc.date.available2024-07-22T08:03:35Z
dc.date.issued2023
dc.description.abstractBackground: Obesity is associated with increased mortality among ovarian cancer and is a poor prognostic factor. There are significant links between the leptin hormone, a product of the obesity gene, and the development of ovarian cancer. Leptin is a vital hormone-like cytokine secreted from adipose tissue and is mainly involved in the maintenance of energy homeostasis. It regulates several intracellular signaling pathways and also interacts with various hormones and energy regulators. It acts as a growth factor by stimulating cell proliferation and differentiation and in this way contributes to cancer cell development. The aim of the study was to investigate the effects of leptin on human ovarian cancer cells. Methods: In this study, the effects of increasing the concentration of leptin were investigated on the cell viability of OVCAR-3 and MDAH-2774 ovarian cancer lines by MTT assay. Moreover, to elucidate the molecular mechanisms of leptin in ovarian cancer cells, changes in the expression levels of 80 cytokines were evaluated after leptin treatment via a human cytokine antibody array. Results: Leptin increases the proliferation of both ovarian cancer cell lines. IL-1 level was increased in OVCAR-3 cells and TGF-β level was increased in MDAH-2774 cells after leptin treatment. A decrease in IL-2, MCP-2/CCL8 and MCP-3/CCL7 levels was detected in both ovarian cancer cell lines with leptin administration. An increase in IL-3 and IL-10 expressions, insulin-like growth factor binding proteins (IGFBP) IGFBP-1, IGFBP-2 and IGFBP-3 levels were detected in both ovarian cancer cell lines with leptin administration. In conclusion; leptin has a proliferative effect on human ovarian cancer cell lines and affects different cytokines in different types of ovarian cancer cells. Copyright 2023 Dincer et al.
dc.identifier.DOI-ID10.7717/peerj.15246
dc.identifier.issn21678359
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/12360
dc.language.isoEnglish
dc.publisherPeerJ Inc.
dc.rightsAll Open Access; Gold Open Access; Green Open Access
dc.subjectcytokine
dc.subjectepidermal growth factor
dc.subjectfibroblast growth factor 6
dc.subjectinterleukin 1
dc.subjectinterleukin 10
dc.subjectinterleukin 12
dc.subjectinterleukin 13
dc.subjectinterleukin 16
dc.subjectinterleukin 2
dc.subjectinterleukin 3
dc.subjectinterleukin 5
dc.subjectinterleukin 8
dc.subjectleptin
dc.subjectmonocyte chemotactic protein 2
dc.subjectmonocyte chemotactic protein 3
dc.subjectplacental growth factor
dc.subjectplatelet derived growth factor BB
dc.subjectsomatomedin binding protein 1
dc.subjectsomatomedin binding protein 2
dc.subjectsomatomedin binding protein 3
dc.subjecttransforming growth factor beta
dc.subjectArticle
dc.subjectcell proliferation
dc.subjectcell viability
dc.subjectcell viability assay
dc.subjectchemoluminescence
dc.subjectcontrolled study
dc.subjectfemale
dc.subjecthuman
dc.subjecthuman cell
dc.subjectMDAH 2774 cell line
dc.subjectMTT assay
dc.subjectobesity
dc.subjectovarian cancer cell line
dc.subjectovary cancer
dc.subjectOVCAR-3 cell line
dc.subjectXTT assay
dc.titleEffects of leptin on the viability of human ovarian cancer cells and changes in cytokine expression levels
dc.typeArticle

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