Comparison of intraperitoneal and intratesticular ozone therapy for the treatment of testicular ischemia-reperfusion injury in rats
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Date
2017
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Abstract
We compare the efficacy of intratesticular ozone therapy with intraperitoneal ozone therapy in an experimental rat model. For this purpose, 24 rats were divided into four groups including sham-operated, torsion/detorsion, torsion/detorsion plus intraperitoneal ozone (O-IP), and torsion/detorsion plus intratesticular ozone (O-IT). The O-IP ozone group received a 4 mg kg-1 intraperitoneal injection of ozone, and the O-IT group received the same injection epididymally. At 4 h after detorsion, the rats were sacrificed and orchiectomy materials were assessed histopathologically. Spermatogenesis in the seminiferous tubules and damage to the Sertoli cells were histopathologically evaluated in the testes using the Johnsen scoring system. i-NOS and e-NOS activities in the testis tissue were also evaluated. Torsion-detorsion caused a decreased Johnsen score and increased apoptosis of spermatogonial and Sertoli cells. Ozone injection prevented increases in Johnsen score and i-NOS level. e-NOS level of the O-IP group was significantly lower than that of the O-IP group, and i-NOS level of the O-IT group was significantly lower than that of the O-IP group. Local ozone therapy is more effective than systemic ozone therapy at improving IRI-related testicular torsion. Our study is the first to show that the efficacy of intratesticular implementation of ozone therapy is higher than that of intraperitoneal ozone therapy. © 2017 AJA, SIMM and SJTU. All rights reserved.
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Animals , Epididymis , Injections , Injections, Intraperitoneal , Male , Nitric Oxide Synthase Type II , Nitric Oxide Synthase Type III , Oxidants, Photochemical , Ozone , Rats , Rats, Wistar , Reperfusion Injury , Sertoli Cells , Spermatic Cord Torsion , Spermatogonia , Testis , nitric oxide synthase , ozone , endothelial nitric oxide synthase , inducible nitric oxide synthase , Nos2 protein, rat , Nos3 protein, rat , ozone , photochemical smog , animal model , animal tissue , Article , assessment of humans , controlled study , drug administration route , enzyme activity , histopathology , intermethod comparison , intraperitoneal ozone therapy , intratesticular ozone therapy , johnsen scoring system , male , nonhuman , orchiectomy , ozone therapy , rat , reperfusion injury , Sertoli cell , spermatogenesis , testis injury , testis torsion , animal , drug effects , epididymis , injection , intraperitoneal drug administration , metabolism , pathology , pharmacology , photochemical smog , reperfusion injury , spermatogonium , testis , vascularization , Wistar rat