Comparison of intraperitoneal and intratesticular ozone therapy for the treatment of testicular ischemia-reperfusion injury in rats

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dc.contributor.authorMete F.
dc.contributor.authorTarhan H.
dc.contributor.authorCelik O.
dc.contributor.authorAkarken I.
dc.contributor.authorVural K.
dc.contributor.authorEkin R.
dc.contributor.authorAydemir I.
dc.contributor.authorIlbey Y.
dc.date.accessioned2024-07-22T08:11:18Z
dc.date.available2024-07-22T08:11:18Z
dc.date.issued2017
dc.description.abstractWe compare the efficacy of intratesticular ozone therapy with intraperitoneal ozone therapy in an experimental rat model. For this purpose, 24 rats were divided into four groups including sham-operated, torsion/detorsion, torsion/detorsion plus intraperitoneal ozone (O-IP), and torsion/detorsion plus intratesticular ozone (O-IT). The O-IP ozone group received a 4 mg kg-1 intraperitoneal injection of ozone, and the O-IT group received the same injection epididymally. At 4 h after detorsion, the rats were sacrificed and orchiectomy materials were assessed histopathologically. Spermatogenesis in the seminiferous tubules and damage to the Sertoli cells were histopathologically evaluated in the testes using the Johnsen scoring system. i-NOS and e-NOS activities in the testis tissue were also evaluated. Torsion-detorsion caused a decreased Johnsen score and increased apoptosis of spermatogonial and Sertoli cells. Ozone injection prevented increases in Johnsen score and i-NOS level. e-NOS level of the O-IP group was significantly lower than that of the O-IP group, and i-NOS level of the O-IT group was significantly lower than that of the O-IP group. Local ozone therapy is more effective than systemic ozone therapy at improving IRI-related testicular torsion. Our study is the first to show that the efficacy of intratesticular implementation of ozone therapy is higher than that of intraperitoneal ozone therapy. © 2017 AJA, SIMM and SJTU. All rights reserved.
dc.identifier.DOI-ID10.4103/1008-682X.171570
dc.identifier.issn1008682X
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15588
dc.language.isoEnglish
dc.publisherMedknow Publications
dc.rightsAll Open Access; Gold Open Access; Green Open Access
dc.subjectAnimals
dc.subjectEpididymis
dc.subjectInjections
dc.subjectInjections, Intraperitoneal
dc.subjectMale
dc.subjectNitric Oxide Synthase Type II
dc.subjectNitric Oxide Synthase Type III
dc.subjectOxidants, Photochemical
dc.subjectOzone
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectReperfusion Injury
dc.subjectSertoli Cells
dc.subjectSpermatic Cord Torsion
dc.subjectSpermatogonia
dc.subjectTestis
dc.subjectnitric oxide synthase
dc.subjectozone
dc.subjectendothelial nitric oxide synthase
dc.subjectinducible nitric oxide synthase
dc.subjectNos2 protein, rat
dc.subjectNos3 protein, rat
dc.subjectozone
dc.subjectphotochemical smog
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectArticle
dc.subjectassessment of humans
dc.subjectcontrolled study
dc.subjectdrug administration route
dc.subjectenzyme activity
dc.subjecthistopathology
dc.subjectintermethod comparison
dc.subjectintraperitoneal ozone therapy
dc.subjectintratesticular ozone therapy
dc.subjectjohnsen scoring system
dc.subjectmale
dc.subjectnonhuman
dc.subjectorchiectomy
dc.subjectozone therapy
dc.subjectrat
dc.subjectreperfusion injury
dc.subjectSertoli cell
dc.subjectspermatogenesis
dc.subjecttestis injury
dc.subjecttestis torsion
dc.subjectanimal
dc.subjectdrug effects
dc.subjectepididymis
dc.subjectinjection
dc.subjectintraperitoneal drug administration
dc.subjectmetabolism
dc.subjectpathology
dc.subjectpharmacology
dc.subjectphotochemical smog
dc.subjectreperfusion injury
dc.subjectspermatogonium
dc.subjecttestis
dc.subjectvascularization
dc.subjectWistar rat
dc.titleComparison of intraperitoneal and intratesticular ozone therapy for the treatment of testicular ischemia-reperfusion injury in rats
dc.typeArticle

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