Blockade of PD-1/PD-L1 Axis May Improve NK-92 CellInhibition Caused by Mesenchymal Stem Cells
| dc.contributor.author | Alper Tunga ÖZDEMİR | |
| dc.contributor.author | Cengiz KIRMAZ | |
| dc.contributor.author | Rabia Bilge Özgül ÖZDEMİR | |
| dc.contributor.author | Mustafa ÖZTATLICI | |
| dc.contributor.author | Mehmet İbrahim TUĞLU | |
| dc.contributor.author | Pınar Kilicarslan SÖNMEZ | |
| dc.contributor.author | Kamil VURAL | |
| dc.contributor.author | Afig BERDELİ | |
| dc.date.accessioned | 2024-07-24T09:14:32Z | |
| dc.date.available | 2024-07-24T09:14:32Z | |
| dc.date.issued | 2021 | |
| dc.description.abstract | Objectives: In this study, it was aimed to investigate how the effects of Mesenchymal stem cells (MSCs) on the anti tumor properties of NK-92 cells change with programmed death-ligand-1 (PD-L1) blocking antibodies. Methods: NK-92 cells were co-cultured with MDA-MB-231 breast tumor cells and MSCs. To evaluate the effect of anti PD-L1 antibodies, cells were cultured for 48 hours with and without the addition of 1, 5, and 10 µg/ml anti PD-L1antibody. IFN-γ, TNF-α, IL-10 and IDO levels of medium supernatants were determined by ELISA. CCK-8 kit was used toevaluate cytotoxic activity. Results: IFN-γ and TNF-α expressions of NK-92 cells co-cultured with MDA-MB-231 increased significantly, but thisincrease was significantly decreased in culture groups with MSCs. IDO expressions increased significantly in co-culturegroups with MSCs only. Cytotoxic effects of NK-92 cells were significantly reduced in culture groups with MSCs. How ever, the suppression effects caused by MSCs improved in the presence of anti-PD-L1 antibodies and in a dose depen dent manner. Conclusion: In our findings, we found that MSCs are a highly effective inhibitors, and the IDO enzyme they secrete mayplay a major role in this. However, the suppressive effects caused by MSCs may be significantly improved by blockingthe PD-1/PD-L1 axis. | |
| dc.identifier.DOI-ID | 10.14744/ejmi.2021.49785 | |
| dc.identifier.uri | http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/26139 | |
| dc.language.iso | eng | |
| dc.title | Blockade of PD-1/PD-L1 Axis May Improve NK-92 CellInhibition Caused by Mesenchymal Stem Cells | |
| dc.type | Araştırma Makalesi |