Novel benzimidazole derivatives: Synthesis, in vitro cytotoxicity, apoptosis and cell cycle studies
| dc.contributor.author | Atmaca H. | |
| dc.contributor.author | İlhan S. | |
| dc.contributor.author | Batır M.B. | |
| dc.contributor.author | Pulat Ç.Ç. | |
| dc.contributor.author | Güner A. | |
| dc.contributor.author | Bektaş H. | |
| dc.date.accessioned | 2025-04-10T11:06:21Z | |
| dc.date.available | 2025-04-10T11:06:21Z | |
| dc.date.issued | 2020 | |
| dc.description.abstract | The aim of the study was to synthesize a new series of benzimidazole derivatives and to investigate the underlying molecular mechanisms of the potential cell cycle inhibition and apoptotic effects against a panel of selected human cancer cell lines along with HEK-293 human embryonic kidney cells. MTT assay was used to evaluate cytotoxic effects. Muse™ Cell Analyzer was used to assess cell cycle progression. Annexin-V/PI staining assay was used for detecting apoptosis. All the synthesized compounds showed a significant cytotoxic effect against cancer cells with the IC50 values between 9.2 and 166.1 μg/mL. Among the tested derivatives, compound 5 showed significant cytotoxic activity against MCF-7, DU-145 and H69AR cancer cells with the IC50 values of 17.8 ± 0.24, 10.2 ± 1.4 and 49.9 ± 0.22 μg/mL respectively. The compounds 5 was also tested on HEK-293 human embryonic kidney cells and found to be safer with lesser cytotoxicity. The results revealed that compound 5 significantly increased cell population in the G2/M-phase which is modulated by a p53 independent mechanism. Compound 5 caused an increase in the percentage of late apoptotic cells in all tested cancer cells in a concentration-dependent manner. Among all synthesized derivatives, compound 5 the bromo-derivative, showed the highest cytotoxic potential, induced G2/M cell cycle arrest and apoptotic cell death in genotypically different human cancer cells. These results suggest that compound 5 might be a promising agent for cancer therapy and further structural modifications of benzimidazole derivatives may create promising anticancer agents. © 2020 Elsevier B.V. | |
| dc.identifier.DOI-ID | 10.1016/j.cbi.2020.109163 | |
| dc.identifier.uri | http://hdl.handle.net/20.500.14701/46654 | |
| dc.publisher | Elsevier Ireland Ltd | |
| dc.title | Novel benzimidazole derivatives: Synthesis, in vitro cytotoxicity, apoptosis and cell cycle studies | |
| dc.type | Article |