Synthesis, characterization and radiolabeling of folic acid modified nanostructured lipid carriers as a contrast agent and drug delivery system
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Date
2017
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Abstract
Nanostructured lipid carriers (NLCs) are the new generation of solid lipid drug delivery systems. Their suitability as contrast agents for gamma scintigraphy is an attracting major attention. The aim of current study was to prepare surface modified nanostructured lipid carrier system for paclitaxel (PTX) with active targeting and imaging functions. In accordance with the purpose of study, PTX loaded nanostructured lipid carriers (NLCs) prepared, modified with a folate derivative and radiolabeled with technetium-99 m tricarbonyl complex (99 mTc(CO)3 +). Cellular incorporation ratios of radiolabeled nanoparticles (99 mTc(CO)3-PTX-NLC) were investigated in vitro on three cancer cell lines. Additionally in vivo animal studies conducted to evaluate biological behavior of 99 mTc(CO)3-PTX-NLC on female Wistar Albino rats. Biodistribution results showed that the folate derivative modified 99 mTc(CO)3-PTX-NLC had considerably higher uptake in folate receptor positive organs. The data obtained from present study could be useful in the design of biodegradable drug carriers of PTX and folate receptor based tumor imaging agents. © 2016 Elsevier Ltd
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Keywords
A549 Cells , Animals , Contrast Media , Drug Carriers , Drug Delivery Systems , Female , Folic Acid , HeLa Cells , Humans , Lipids , MCF-7 Cells , Microscopy, Fluorescence , Nanostructures , Paclitaxel , Rats , Rats, Wistar , Technetium , Tissue Distribution , Cell culture , Nuclear medicine , Targeted drug delivery , Technetium compounds , folate receptor , folic acid , paclitaxel , radioisotope , solid lipid nanoparticle , technetium tricarbonyl complex tc 99m , unclassified drug , contrast medium , drug carrier , folic acid , lipid , nanomaterial , paclitaxel , technetium , Biodistributions , Nanostructured lipid carriers , Paclitaxel , Radiolabeling , Technetium-99m , animal experiment , Article , cell viability , controlled study , cytotoxicity , dispersity , drug absorption , drug delivery system , drug distribution , drug purity , drug synthesis , female , fluorescence microscopy , human , human cell , isotope labeling , nanotechnology , nonhuman , particle size , priority journal , protein expression , proton nuclear magnetic resonance , rat , tissue distribution , transmission electron microscopy , Wistar rat , zeta potential , A-549 cell line , animal , chemistry , HeLa cell line , MCF-7 cell line , synthesis , Controlled drug delivery