N-acetylcysteine ameliorates nitrosative stress on radiation-inducible damage in rat liver
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Date
2011
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Abstract
Purpose: The present study was designed to investigate the potential radioprotective effects of N-acetylcysteine (NAC) on radiation-induced nitrosative stress caused by gamma irradiation (single dose, 6 Gy) in rat liver. Methods: The rats (n=40) were divided randomly and equally into 4 groups: Control (C), Radiation (R), R+NAC (received irradiation and 1,000 mg/kg of NAC) and R+WR-2721 (received irradiation and 200 mg/kg of WR-2721). Liver tissue of each animal was harvested and utilized for 3-nitrotyrosine (3-NT) detection using high-performance liquid chromatography-ultraviolet (HPLC-UV) system. Results: In the R rats, 3-NT levels significantly increased when compared to those of the C rats (p<0.05). There were no significant differences in the 3-NT levels among R+NAC and R+WR-2721 rats. Histologically examined liver tissue samples showed no obvious differences. Conclusion: The present study suggests that irradiation has a negative effect on the cellular proteins by enhancing 3-NT formation. The prophylactic use of NAC seems to reduce the nitrosative damage during radiotherapy. © 2011 Zerbinis Medical Publications.
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Acetylcysteine , Amifostine , Animals , Female , Liver , Nitric Oxide , Radiation-Protective Agents , Rats , Rats, Wistar , Tyrosine , 3 nitrotyrosine , acetylcysteine , amifostine , tyrosine , animal cell , animal experiment , animal tissue , article , controlled study , gamma irradiation , high performance liquid chromatography , liver cell , liver histology , liver injury , nitrosative stress , nonhuman , radiation dose , radiation protection , rat , treatment outcome