The potential role of the NEK6, AURKA, AURKB, and PAK1 genes in adenomatous colorectal polyps and colorectal adenocarcinoma
No Thumbnail Available
Date
2016
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Colorectal adenomatous polyp (CRAP) is a major risk factor for the development of sporadic colorectal cancer (CRC). Histone modifications are one of the epigenetic mechanisms that may have key roles in the carcinogenesis of CRC. The objective of the present study is to investigate the alternations in the defined histone modification gene expression profiles in patients with CRAP and CRC. Histone modification enzyme key gene expressions of the CRC, CRAP, and control groups were evaluated and compared using the reverse transcription PCR (RT-PCR) array method. Gene expression analysis was performed in the CRAP group after dividing the patients into subgroups according to the polyp diameter, pathological results, and morphological parameters which are risk factors for developing CRC in patients with CRAP. PAK1, NEK6, AURKA, AURKB, HDAC1, and HDAC7 were significantly more overexpressed in CRC subjects compared to the controls (p < 0.05). PAK1, NEK6, AURKA, AURKB, and HDAC1 were significantly more overexpressed in the CRAP group compared to the controls (p < 0.005). There were no significant differences between the CRAP and CRC groups with regards to PAK1, NEK6, AURKA, or AURKB gene overexpression. PAK1, NEK6, AURKA, and AURKB were significantly in correlation with the polyp diameter as they were more overexpressed in polyps with larger diameters. In conclusion, overexpressions of NEK6, AURKA, AURKB, and PAK1 genes can be used as predictive markers to decide the colonoscopic surveillance intervals after the polypectomy procedure especially in polyps with larger diameters. © 2015, International Society of Oncology and BioMarkers (ISOBM).
Description
Keywords
Adenocarcinoma , Adenomatous Polyposis Coli , Adult , Aged , Aurora Kinase A , Aurora Kinase B , Colorectal Neoplasms , Female , Humans , Male , Middle Aged , NIMA-Related Kinases , p21-Activated Kinases , aurora A kinase , aurora B kinase , carcinoembryonic antigen , histone deacetylase 1 , histone deacetylase 7 , p21 activated kinase 1 , aurora A kinase , aurora B kinase , NEK6 protein, human , NIMA related kinase , p21 activated kinase , PAK1 protein, human , adenomatous colorectal polyp , adult , Article , AURKA gene , AURKB gene , cancer growth , cancer risk , cancer staging , colorectal carcinoma , colorectal polyp , comparative study , controlled study , female , gene , gene expression profiling , gene overexpression , general aspects of disease , genetic association , HDAC1 gene , HDAC7 gene , high risk population , histone modification , histopathology , human , human cell , human tissue , major clinical study , male , middle aged , morphology , NEK6 gene , PAK1 gene , pathophysiology , priority journal , reverse transcription polymerase chain reaction , tumor localization , adenocarcinoma , aged , colon polyposis , colorectal tumor , genetics , pathology , physiology