The Effect of Resveratrol and Quercetin on Epithelial-Mesenchymal Transition in Pancreatic Cancer Stem Cell
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Date
2020
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Abstract
Resveratrol and quercetin are phytochemicals that are found in a variety of plants. The aim of this study was to investigate the effect of resveratrol and quercetin on epithelial-mesenchymal transition (EMT) of CD133+ and CD133− pancreatic cancer cells. Cancer stem cells (CD133+ cells) were obtained from the PANC-1 cells by the MiniMACS system. CD133+ and CD133− PANC-1 cells were treated with different concentrations (5, 10, 25, 50, and 100 µM) of resveratrol and quercetin. Cell growth and cytotoxicity were evaluated by MTT assay. Anticancer and anti-metastatic properties of resveratrol and quercetin were determined by immunocytochemistry using antibodies (ACTA-2, IL-1β, N-cadherin, TNF-α, and vimentin). The immunostaining intensity of CD133+ cells was stronger than CD133− cells. ACTA-2, IL-1β, and N-cadherin immunoreactivities were significantly decreased, whereas TNF-α and vimentin immunoreactivities significantly increased in quercetin-treated CD133+ cells. Moreover, N-cadherin and TNF-α immunoreactivities significantly decreased in resveratrol-treated CD133+ cells. The reduction in N-cadherin and ACTA-2 immunoreactivities was higher than the increase in vimentin immunoreactivity, quercetin could prevent EMT to a greater extent than resveratrol in pancreatic cancer stem cells because of the reduced expression of N-cadherin. Quercetin could be more effective in inhibiting metastasis compared to resveratrol. © 2020, © 2019 Taylor & Francis Group, LLC.
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Antigens, CD , Antineoplastic Agents , Cadherins , Cell Line, Tumor , Cell Proliferation , Cell Survival , Epithelial-Mesenchymal Transition , Humans , Neoplastic Stem Cells , Pancreatic Neoplasms , Quercetin , Resveratrol , Tumor Necrosis Factor-alpha , Vimentin , acta 2 protein , biological marker , CD133 antigen , interleukin 1 , interleukin 1beta , nerve cell adhesion molecule , quercetin , resveratrol , tumor necrosis factor , unclassified drug , vimentin , antineoplastic agent , cadherin , leukocyte antigen , quercetin , resveratrol , tumor necrosis factor , vimentin , antineoplastic activity , Article , cancer stem cell , cell growth , cell viability , concentration response , controlled study , drug cytotoxicity , drug effect , epithelial mesenchymal transition , human , human cell , immunocytochemistry , immunoreactivity , metastasis inhibition , MTT assay , PANC-1 cell line , pancreas cancer , cancer stem cell , cell proliferation , cell survival , epithelial mesenchymal transition , metabolism , pancreas tumor , pathology , tumor cell line