The Effect of Resveratrol and Quercetin on Epithelial-Mesenchymal Transition in Pancreatic Cancer Stem Cell

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dc.contributor.authorHoca M.
dc.contributor.authorBecer E.
dc.contributor.authorKabadayı H.
dc.contributor.authorYücecan S.
dc.contributor.authorVatansever H.S.
dc.date.accessioned2024-07-22T08:07:04Z
dc.date.available2024-07-22T08:07:04Z
dc.date.issued2020
dc.description.abstractResveratrol and quercetin are phytochemicals that are found in a variety of plants. The aim of this study was to investigate the effect of resveratrol and quercetin on epithelial-mesenchymal transition (EMT) of CD133+ and CD133− pancreatic cancer cells. Cancer stem cells (CD133+ cells) were obtained from the PANC-1 cells by the MiniMACS system. CD133+ and CD133− PANC-1 cells were treated with different concentrations (5, 10, 25, 50, and 100 µM) of resveratrol and quercetin. Cell growth and cytotoxicity were evaluated by MTT assay. Anticancer and anti-metastatic properties of resveratrol and quercetin were determined by immunocytochemistry using antibodies (ACTA-2, IL-1β, N-cadherin, TNF-α, and vimentin). The immunostaining intensity of CD133+ cells was stronger than CD133− cells. ACTA-2, IL-1β, and N-cadherin immunoreactivities were significantly decreased, whereas TNF-α and vimentin immunoreactivities significantly increased in quercetin-treated CD133+ cells. Moreover, N-cadherin and TNF-α immunoreactivities significantly decreased in resveratrol-treated CD133+ cells. The reduction in N-cadherin and ACTA-2 immunoreactivities was higher than the increase in vimentin immunoreactivity, quercetin could prevent EMT to a greater extent than resveratrol in pancreatic cancer stem cells because of the reduced expression of N-cadherin. Quercetin could be more effective in inhibiting metastasis compared to resveratrol. © 2020, © 2019 Taylor & Francis Group, LLC.
dc.identifier.DOI-ID10.1080/01635581.2019.1670853
dc.identifier.issn01635581
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/13814
dc.language.isoEnglish
dc.publisherRoutledge
dc.subjectAntigens, CD
dc.subjectAntineoplastic Agents
dc.subjectCadherins
dc.subjectCell Line, Tumor
dc.subjectCell Proliferation
dc.subjectCell Survival
dc.subjectEpithelial-Mesenchymal Transition
dc.subjectHumans
dc.subjectNeoplastic Stem Cells
dc.subjectPancreatic Neoplasms
dc.subjectQuercetin
dc.subjectResveratrol
dc.subjectTumor Necrosis Factor-alpha
dc.subjectVimentin
dc.subjectacta 2 protein
dc.subjectbiological marker
dc.subjectCD133 antigen
dc.subjectinterleukin 1
dc.subjectinterleukin 1beta
dc.subjectnerve cell adhesion molecule
dc.subjectquercetin
dc.subjectresveratrol
dc.subjecttumor necrosis factor
dc.subjectunclassified drug
dc.subjectvimentin
dc.subjectantineoplastic agent
dc.subjectcadherin
dc.subjectleukocyte antigen
dc.subjectquercetin
dc.subjectresveratrol
dc.subjecttumor necrosis factor
dc.subjectvimentin
dc.subjectantineoplastic activity
dc.subjectArticle
dc.subjectcancer stem cell
dc.subjectcell growth
dc.subjectcell viability
dc.subjectconcentration response
dc.subjectcontrolled study
dc.subjectdrug cytotoxicity
dc.subjectdrug effect
dc.subjectepithelial mesenchymal transition
dc.subjecthuman
dc.subjecthuman cell
dc.subjectimmunocytochemistry
dc.subjectimmunoreactivity
dc.subjectmetastasis inhibition
dc.subjectMTT assay
dc.subjectPANC-1 cell line
dc.subjectpancreas cancer
dc.subjectcancer stem cell
dc.subjectcell proliferation
dc.subjectcell survival
dc.subjectepithelial mesenchymal transition
dc.subjectmetabolism
dc.subjectpancreas tumor
dc.subjectpathology
dc.subjecttumor cell line
dc.titleThe Effect of Resveratrol and Quercetin on Epithelial-Mesenchymal Transition in Pancreatic Cancer Stem Cell
dc.typeArticle

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