Portable quantification of silver ion by using personal glucose meter (PGM) and magnetite cross-linked invertase aggregates (MCLIA)
No Thumbnail Available
Date
2022
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Heavy metal detection is critical due to its harmful effects on human health and the ecosystem. Enzyme-based platforms attract attention for heavy metal detection such as silver, a toxic metal, due to being small, portable, and requiring only essential equipment compared with the basic analytical methods. In this study, magnetic cross-linked invertase aggregates (MCLIA) were developed for the first time as an enzyme-based signaling platform to detect Ag+ using a personal glucose meter (PGM). EDX, FTIR, and VSM results depicted that MCLIA was successfully developed and exhibits super-paramagnetism. In addition, MCLIA selectively detected the Ag+ at a sensitivity of 1.2 inhibition rate/μM in a linear range from 5 to 70 μM with a detection limit of 4.6 μM and IC50 value of 42.3 μM. These findings strongly indicate that MCLIA is applicable as a signal platform for portable quantification of other analytes that inhibits the invertase enzyme. © 2021 Elsevier Inc.
Description
Keywords
Biosensing Techniques , Blood Glucose Self-Monitoring , Glucose Oxidase , Ions , Silver , Aggregates , Chemical detection , Enzyme inhibition , Magnetite , Metal ions , Silver , cobalt , copper , magnetite nanoparticle , nickel , silver , zinc , glucose oxidase , ion , silver , Ag + , Ag+ detection , Enzyme aggregate , Harmful effects , Heavy metals detection , Human health , Invertase inhibition , Personal glucose meter , Portable quantification , Silver ions , analytic method , analytical parameters , Article , calibration curve , chemical composition , chemical structure , energy dispersive X ray spectroscopy , enzyme activity , enzyme inhibition , Fourier transform infrared spectroscopy , IC50 , immobilization , limit of detection , linearity , magnetite cross linked invertase aggregate method , scanning electron microscopy , sensitivity , vibrating sample magnetometry , blood glucose monitoring , genetic procedures , metabolism , Glucose