Determination of apoptosis and cell cycle modulators (p16, p21, p27, p53, BCL-2, Bax, BCL-xL, and cyclin D1) in thyroid follicular carcinoma, follicular adenoma, and adenomatous nodules via a tissue microarray method
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Date
2015
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Abstract
Background/aim: To identify the role of gene products associated with apoptosis and cell cycle in the pathogenesis of thyroid follicularneoplasm. Materials and methods: Thirty follicular adenomas (FAs), 16 follicular carcinomas (FCs), and 20 adenomatous nodules (ANs) wereinvestigated with immunohistochemical staining of p16, p21, p27, p53, Bcl-2, Bax, Bcl-xL, and cyclin D1 via a tissue microarray method.Results: Bcl-2 showed a significant difference between the benign groups (AN and FA) and the malignant group (FC). Bax wassignificantly higher in the FC group. p53 was lowest in the AN group and highest in the FC group with significant differences betweenthe groups. p16 was significantly higher in the FC group than in the other groups. There was a significant difference between the ANgroup and neoplastic lesions in terms of p21 staining. The number of cases with positive p27 was lower in the AN group than theneoplastic groups. There was no significant difference in terms of Bcl-xL and cyclin D1. Conclusion: Cell cycle modulators, led by the Bcl-2 family, played an important role in the pathogenesis of thyroid follicular neoplasm,and p53, p16, and p21 in particular played a role in the carcinogenesis of FC.