Hydrophobic nano-carrier for lysozyme adsorption

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dc.contributor.authorAltunbas, C
dc.contributor.authorUral, FZ
dc.contributor.authorUygun, M
dc.contributor.authorAvcibasi, N
dc.contributor.authorAvcibasi, U
dc.contributor.authorUygun, DA
dc.contributor.authorAkgöl, S
dc.date.accessioned2024-07-18T12:03:26Z
dc.date.available2024-07-18T12:03:26Z
dc.description.abstractIn this work, poly(HEMA-APH) nanoparticles were synthesized by surfactant-free emulsion polymerization technique. Magnetic behaviour was introduced by simple addition of Fe3O4 into the polymerization medium. Characterization of the nanoparticle was carried out by FTIR, ESR, SEM, AFM and EDX analyses. These synthesized magnetic nanoparticles were used for adsorption of lysozyme. For this purpose, adsorption conditions were optimized and maximum lysozyme binding capacity was found to be 278.8 mg g(-1) polymer in pH 7.0 phosphate buffer at 25(a similar to)C. Desorption and reusability properties of the nanoparticles were investigated and lysozyme adsorption efficiency did not change significantly at the end of the 10 successive reuses.
dc.identifier.issn0250-4707
dc.identifier.other0973-7669
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/9123
dc.language.isoEnglish
dc.publisherINDIAN ACAD SCIENCES
dc.subjectMETAL CHELATE AFFINITY
dc.subjectINTERACTION CHROMATOGRAPHY
dc.subjectMAGNETIC NANOPARTICLES
dc.subjectMETHACRYLATE) BEADS
dc.subjectIMMOBILIZED ENZYMES
dc.subjectSOLVOPHOBIC THEORY
dc.subjectPROTEINS
dc.subjectPURIFICATION
dc.subjectLIPASE
dc.titleHydrophobic nano-carrier for lysozyme adsorption
dc.typeArticle

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