Low Mononuclear Cell IL-18 and IL-27 Response in Children: Susceptibility to Tuberculosis Infection after Contact

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dc.contributor.authorKarabacak H.A.
dc.contributor.authorYilmaz O.
dc.contributor.authorTuglu I.
dc.contributor.authorTaneli F.
dc.contributor.authorSurucuoglu S.
dc.contributor.authorKanik E.T.
dc.contributor.authorOzkutuk N.
dc.contributor.authorGozukara C.
dc.contributor.authorOzkut M.M.
dc.contributor.authorTurkeli A.
dc.contributor.authorYuksel H.
dc.date.accessioned2025-04-10T11:07:31Z
dc.date.available2025-04-10T11:07:31Z
dc.date.issued2019
dc.description.abstractBackground Identification of the immune response against tuberculosis is vital to develop new diagnostic and therapeutic modalities. The objective of this study was to determine IL (interleukin)-18 and IL-27 responses of peripheral blood mononuclear cells to early secreted antigen (ESAT-6) and culture filtrate protein-10 (CFP-10) stimulation in children with a (+) or (-) tuberculin skin test (TST) with in-house tuberculosis contact. Methods We enrolled 40 children aged 1 to 5 years who had an in-house contact with a tuberculous adult. Blood samples were obtained from all children for QuantiFERON tuberculosis (TB) gold in tube (QFT-GIT), and peripheral blood mononuclear blood cell culture tests. The subjects were grouped as TST (-) QFT-GIT (-), TST (+) QFT-GIT (-), and TST (+) QFT-GIT (+). Supernatant of peripheral blood mononuclear cell culture was separated with and without stimulation of ESAT-6 and CFP-10, and IL-18 and IL-27 levels were measured with enzyme linked immunoassay (ELISA) test. Results The study group included 22 boys and 18 girls with mean age 4.25 ± 0.9 years. IL-18 and IL-27 levels were statistically significant in ESAT-6/CFP-10-stimulated supernatants of peripheral blood mononuclear cell (PBMC) samples among the three groups (p = 0.000, p = 0.007, respectively). IL-18 levels between the TST (-) QFT-GIT (-) and TST (+) QFT-GIT (+) groups were significantly different (p = 0.026). Both IL-18 and IL-27 levels were significantly different between ESAT-6/CFP-10 stimulated PBMC supernatants of TST (-) QFT-GIT (-) and TST (+) QFT-GIT (-) groups (p = 0.000, p = 0.003, respectively). Conclusion Low IL-18 and IL-27 responses of peripheral blood mononuclear cells in children with Bacillus Calmette-Guérin (BCG) vaccine may play a role in Mycobacterium tuberculosis infection after in-house contact. © 2019 by Georg Thieme Verlag KG, Stuttgart. New York.
dc.identifier.DOI-ID10.1055/s-0039-1679875
dc.identifier.urihttp://hdl.handle.net/20.500.14701/47411
dc.publisherGeorg Thieme Verlag
dc.titleLow Mononuclear Cell IL-18 and IL-27 Response in Children: Susceptibility to Tuberculosis Infection after Contact
dc.typeArticle

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