Immunomodulatory Effects of MDA-MB-231-derived Exosome Mimetic Nanovesicles on CD4+ T Cell Line

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dc.contributor.authorOztatlici M.
dc.contributor.authorOzdemir A.T.
dc.contributor.authorOztatlici H.
dc.contributor.authorKucukhuyuk S.
dc.contributor.authorOzgul Ozdemir R.B.
dc.contributor.authorOrhan H.
dc.contributor.authorOzbilgin K.
dc.date.accessioned2025-04-10T11:02:56Z
dc.date.available2025-04-10T11:02:56Z
dc.date.issued2024
dc.description.abstractObjectives: The aim of this study is to investigate the immunomodulatory effects of MDA-MB-231 cells or MDA-MB-231-derived exosome-mimetic nanovesicles (NVs) on CD4+ Jurkat cells. Methods: NVs were produced by the breakdown of MDA-MB-231 cells and the characterization of generated NVs were performed by using direct-ELISA and Flow cytometry methods. We co-cultured CD4+ Jurkat cells with MDA-MB-231 cells or MDA-MB-231-derived NVs for 48 h. Subsequently, expressions of pro-inflammatory and anti-inflammatory cy-tokines, and related transcription factors of CD4+ Jurkat cells were evaluated by qPCR method. Results: Clustering, which is the indicator of activation, was not seen in the CD4+ Jurkat cells co-cultured with MDA-MB-231 cells. However, CD4+ Jurkat cell clusters were observed in the co-culture experiments with all NV concentrations. In addition, it was determined that the expressions of pro-inflammatory cytokines significantly increased while the expressions of anti-inflammatory cytokines was dramatically decreased in the NV-treated groups. On the contrary, opposite results were obtained in CD4+ Jurkat cells co-cultured with MDA-MB-231 cells. Moreover, TNF-α and Gata3 expressions were decreased in all groups. Conclusion: These preliminary findings from in-vitro experiments suggested that NVs could be a potential tool in cancer immunotherapy, but our data need to be supported by more comprehensive studies. © 2024 by Eurasian Journal of Medicine and Oncology.
dc.identifier.DOI-ID10.14744/ejmo.2024.64067
dc.identifier.urihttp://hdl.handle.net/20.500.14701/44396
dc.publisherKare Publishing
dc.titleImmunomodulatory Effects of MDA-MB-231-derived Exosome Mimetic Nanovesicles on CD4+ T Cell Line
dc.typeArticle

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