Qualitative Analysis of Alfalfa Seed Methanol Extract by GC-MS and Determination of Antioxidant Properties
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2019
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Abstract
Alfalfa plant has a great importance for agriculture in Turkey and throughout the world. Antioxidantactivities (DPPH and NO scavenging effects) have been studied from the methanol extraction of theseeds, and organic volatile molecule contents have been analyzed by GC-MS. Physical and chemicalanalysis of the seed were also carried out to elucidate the structure. As a result of the experiments, aconcentration-dependent increase was observed in DPPH and NO scavenging (%) activities from the 0.5,1 and 2 mg/ml methanol extract concentrations of alfalfa seeds. In GC-MS analysis, it was found thatsqualene, a pharmacologically active molecule, is present in alfalfa seeds. As a result of chemical andphysical analyzes, it was determined that the crude protein content is 33.79% and crude oil is 8.11%.Although alfalfa is widely used in agriculture and as animal bait, this study shows that alfalfa seeds arealso pharmacologically crucial for containing rich molecules.