Evaluation of the performance of MALDI-TOF MS and DNA sequence analysis in the identification of mycobacteria species

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dc.contributor.authorAkyar, I
dc.contributor.authorÇavusoglu, C
dc.contributor.authorAyas, M
dc.contributor.authorSürücüoglu, S
dc.contributor.authorIlki, A
dc.contributor.authorKaya, DE
dc.contributor.authorBesli, Y
dc.date.accessioned2024-07-18T12:05:49Z
dc.date.available2024-07-18T12:05:49Z
dc.description.abstractBackground/aim: Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry is an alternative way of identifying mycobacteria via the analysis of biomolecules. It is being increasingly used in routine microbiology practice since it permits early, rapid, and cost-effective identification of pathogens of clinical importance. In this study, we aimed to evaluate the efficacy of phenotypic identification of mycobacteria by the MALDI-TOF MS MBT Mycobacteria Library (ML) 4.0 (Bruker, Daltonics) compared to standard sequence analysis. Materials and methods: A total of 155 Mycobacterium clinical and external quality control isolates, comprising nontuberculous mycobacteria (NTM) (n = 95) and the Mycobacterium tuberculosis complex (MTC) (n = 60), were included in the study. Results: Identification by MBT ML4.0 was correctly performed in 100% of MTC and in 91% of NTM isolates. All of the MTC isolates were correctly differentiated from NTM isolates. Conclusion: Based on our results, MBT ML4.0 may be used reliably to identify both NTM and MTC.
dc.identifier.issn1300-0144
dc.identifier.other1303-6165
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/10011
dc.language.isoEnglish
dc.publisherTubitak Scientific & Technological Research Council Turkey
dc.subjectDESORPTION IONIZATION-TIME
dc.subjectFLIGHT MASS-SPECTROMETRY
dc.subjectSP NOV.
dc.subjectSUBSP ABSCESSUS
dc.subjectDISEASE
dc.subjectINFECTION
dc.subjectCHIMERA
dc.subjectRELAPSE
dc.subjectMEMBERS
dc.titleEvaluation of the performance of MALDI-TOF MS and DNA sequence analysis in the identification of mycobacteria species
dc.typeArticle

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