Photostimulation of osteogenic differentiation on silk scaffolds by plasma arc light source
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Date
2018
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Abstract
Low-level laser therapy (LLLT) has been used for more than 30 years to heal wounds. In recent years, LLLT or photostimulation has been indicated as an effective tool for regenerative and dental medicine by using monochromatic light. The aim of this study is to indicate the usability of plasma arc light source for bone regeneration. This is why we used polychromatic light source providing effective wavelengths in the range of 590–1500 nm for cellular response and investigated photostimulation effects on osteogenic differentiation of human mesenchymal stem cells (hMSCs) seeded on 3D silk scaffolds. Cellular responses were examined by using cell culture methods in terms of proliferation, differentiation, and morphological analyses. The results showed that photostimulation with a polychromatic light source (applied for 5 min from the 3rd day after seeding up to the 28th day in 2-day intervals with 92-mW/cm2 power from 10-cm distance to the cells) enhanced osteogenic differentiation of hMSCs according to higher alkaline phosphatase (ALP) activity, collagen and calcium content, osteogenic gene expressions, and matrix mineralization. In conclusion, we suggest that the plasma arc light source that was used here has a great potential for bone regeneration. © 2017, Springer-Verlag London Ltd., part of Springer Nature.
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Keywords
Alkaline Phosphatase , Bone Regeneration , Calcium , Cell Culture Techniques , Cell Differentiation , Cell Proliferation , Cells, Cultured , Collagen , Humans , Infrared Rays , Low-Level Light Therapy , Mesenchymal Stromal Cells , Osteogenesis , Silk , Tissue Scaffolds , alkaline phosphatase , calcium , collagen , DNA , silk fibroin , alkaline phosphatase , calcium , collagen , silk , Article , bone development , bone mineralization , bone regeneration , cell differentiation , cell proliferation , cell structure , cell viability , confocal microscopy , controlled study , energy dispersive X ray spectroscopy , enzyme activity , gene expression , human , human cell , light , mesenchymal stem cell , plasma arc light source , priority journal , reverse transcription polymerase chain reaction , scanning electron microscopy , stem cell culture , bone development , cell culture , cell culture technique , cell differentiation , cytology , infrared radiation , low level laser therapy , mesenchymal stroma cell , metabolism , physiology , radiation response , tissue scaffold , ultrastructure